Down-regulation of guanylate binding protein 1 causes mitochondrial dysfunction and cellular senescence in macrophages

Xiaoxue Qiu, Hong Guo, Junshu Yang, Yinduo Ji, Chia Shan Wu, Xiaoli Chen

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18 Scopus citations


Macrophage polarization is tightly associated with its metabolic reprograming and immune dysfunction. However, the intracellular molecules/pathways that connect these alterations in inflammatory macrophages remain largely unidentified. Herein, we explored the role of guanylate binding protein 1 (Gbp1), an intracellular anti-microbial protein, in regulating polarization, metabolic reprogramming, and cellular aging of macrophages. We showed that Gbp1 expression in inguinal white adipose tissue is significantly decreased in high-fat diet -fed and aged mice. Gbp1 expression is significantly induced by IFNγ and LPS in macrophages but not adipocytes. Downregulation of Gbp1 expression causes macrophage polarization towards a pro-inflammatory phenotype. Gbp1 knockdown (Kd) macrophages have impaired mitochondrial respiratory function, which is further supported by down-regulation of genes encoding electron transport chain components and genes involved in fatty acid oxidation and mitochondrial function. Moreover, we observed Gbp1 is localized in both cytosol and mitochondrial fraction, and Gbp1 Kd macrophages display decreased mitophagy activity. More interestingly, Gbp1 Kd macrophages undergo senescence as evidenced by increased activation of AMPK-p53 pathway and positive staining of β-galactosidase. These observations suggest that Gbp1 may play an important role in protecting against mitochondrial dysfunction and preserving immune function of macrophages during inflammatory stress and aging.

Original languageEnglish (US)
Article number1679
JournalScientific reports
Issue number1
StatePublished - Dec 1 2018

Bibliographical note

Funding Information:
We thank Dr. Rocio Foncea and Dr. David Bernlohr from the Department of Biochemistry, Molecular Biology and Biophysics at the University of Minnesota for technical assistance in measuring cellular respiration. We also thank Dr. Yuxiang Sun from the Department of Nutrition and Food Science, Texas A&M University for kindly providing adipose tissue from aged mice. The project described was supported by grant (P30DK050456) from the National Institute of Diabetes and Digestive and Kidney Diseases and NIDDK-funded Minnesota Obesity Center. General Mills Foundation to X.C., and Tarleton Fellowship to X.Q and CVM Research Office UMN Ag Experiment Station General Ag Research Fund to J.Y.

Publisher Copyright:
© 2018 The Author(s).


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