Doubling time α-aminoisobutyrate transport and calcium exchange in cultured fibroblasts from cystic fibrosis and control subjects

Burton L. Shapiro; Robert J. Feigal; Nancy J. Laible; Michelle H Biros; Warren J. Warwick

doi:10.1016/0009-8981(78)90035-9

Doubling time α-aminoisobutyrate transport and calcium exchange in cultured fibroblasts from cystic fibrosis and control subjects

Burton L. Shapiro, Robert J. Feigal, Nancy J. Laible, Michelle H Biros, Warren J. Warwick

Emergency Medicine

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Abstract

Population doubling time, kinetics of transport of α-aminoisobutyrate (AIB) and calcium (Ca) exchange were studied in skin fibroblast monolayers obtained from 5 subjects with cystic fibrosis (CF) and 5 age- and sex-matched controls. Population doubling time as estimated from cell count, protein and DNA was no different in the two groups. K_M, V_max, maximal uptake and time of half maximal uptake of AIB were no different in the two groups. Intracellular Ca pool size based on exchange of ⁴⁵Ca with unlabelled Ca was significantly greater in monolayers from CF subjects.

Original language	English (US)
Pages (from-to)	125-131
Number of pages	7
Journal	Clinica Chimica Acta
Volume	82
Issue number	1-2
DOIs	https://doi.org/10.1016/0009-8981(78)90035-9
State	Published - Jan 2 1978

Bibliographical note

Funding Information:
This work was supported by a State of Minnesota Special Allocation for Cystic Fibrosis Research, NIH grant 5 TO1 DE 00005 and the Cystic Fibrosis Foundation.

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title = "Doubling time α-aminoisobutyrate transport and calcium exchange in cultured fibroblasts from cystic fibrosis and control subjects",

abstract = "Population doubling time, kinetics of transport of α-aminoisobutyrate (AIB) and calcium (Ca) exchange were studied in skin fibroblast monolayers obtained from 5 subjects with cystic fibrosis (CF) and 5 age- and sex-matched controls. Population doubling time as estimated from cell count, protein and DNA was no different in the two groups. KM, Vmax, maximal uptake and time of half maximal uptake of AIB were no different in the two groups. Intracellular Ca pool size based on exchange of 45Ca with unlabelled Ca was significantly greater in monolayers from CF subjects.",

author = "Shapiro, {Burton L.} and Feigal, {Robert J.} and Laible, {Nancy J.} and Biros, {Michelle H} and Warwick, {Warren J.}",

note = "Funding Information: This work was supported by a State of Minnesota Special Allocation for Cystic Fibrosis Research, NIH grant 5 TO1 DE 00005 and the Cystic Fibrosis Foundation.",

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T1 - Doubling time α-aminoisobutyrate transport and calcium exchange in cultured fibroblasts from cystic fibrosis and control subjects

AU - Shapiro, Burton L.

AU - Feigal, Robert J.

AU - Laible, Nancy J.

AU - Biros, Michelle H

AU - Warwick, Warren J.

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PY - 1978/1/2

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N2 - Population doubling time, kinetics of transport of α-aminoisobutyrate (AIB) and calcium (Ca) exchange were studied in skin fibroblast monolayers obtained from 5 subjects with cystic fibrosis (CF) and 5 age- and sex-matched controls. Population doubling time as estimated from cell count, protein and DNA was no different in the two groups. KM, Vmax, maximal uptake and time of half maximal uptake of AIB were no different in the two groups. Intracellular Ca pool size based on exchange of 45Ca with unlabelled Ca was significantly greater in monolayers from CF subjects.

AB - Population doubling time, kinetics of transport of α-aminoisobutyrate (AIB) and calcium (Ca) exchange were studied in skin fibroblast monolayers obtained from 5 subjects with cystic fibrosis (CF) and 5 age- and sex-matched controls. Population doubling time as estimated from cell count, protein and DNA was no different in the two groups. KM, Vmax, maximal uptake and time of half maximal uptake of AIB were no different in the two groups. Intracellular Ca pool size based on exchange of 45Ca with unlabelled Ca was significantly greater in monolayers from CF subjects.

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Doubling time α-aminoisobutyrate transport and calcium exchange in cultured fibroblasts from cystic fibrosis and control subjects

Abstract

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