Double-cross hydrostatic pressure sample injection for chip CE: Variable sample plug volume and minimum number of electrodes

Yong Luo, Dapeng Wu, Shaojiang Zeng, Hongwei Gai, Zhicheng Long, Zheng Shen, Zhongpeng Dai, Jianhua Qin, Bingcheng Lin

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

A novel sample injection method for chip CE was presented. This injection method uses hydrostatic pressure, generated by emptying the sample waste reservoir, for sample loading and electrokinetic force for dispensing. The injection was performed on a double-cross microchip. One cross, created by the sample and separation channels, is used for formation of a sample plug. Another cross, formed by the sample and controlling channels, is used for plug control. By varying the electric field in the controlling channel, the sample plug volume can be linearly adjusted. Hydrostatic pressure takes advantage of its ease of generation on a microfluidic chip, without any electrode or external pressure pump, thus allowing a sample injection with a minimum number of electrodes. The potential of this injection method was demonstrated by a four-separation-channel chip CE system. In this system, parallel sample separation can be achieved with only two electrodes, which is otherwise impossible with conventional injection methods. Hydrostatic pressure maintains the sample composition during the sample loading, allowing the injection to be free of injection bias.

Original languageEnglish (US)
Pages (from-to)6074-6080
Number of pages7
JournalAnalytical chemistry
Volume78
Issue number17
DOIs
StatePublished - Sep 1 2006

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