Dose-dependent cell growth in response to concentration modulated patterns of FGF-2 printed on fibrin

Eric D. Miller; Gregory W. Fisher; Lee E. Weiss; Lynn M. Walker; Phil G. Campbell

doi:10.1016/j.biomaterials.2005.10.021

Dose-dependent cell growth in response to concentration modulated patterns of FGF-2 printed on fibrin

Eric D. Miller, Gregory W. Fisher, Lee E. Weiss, Lynn M. Walker, Phil G. Campbell

Research output: Contribution to journal › Article › peer-review

101 Scopus citations

Abstract

Immobilized patterns of unmodified fibroblast growth factor-2 (FGF-2), with varying surface concentrations, were inkjet printed onto physiologically relevant fibrin substrates. Printed patterns were characterized using iodinated FGF-2 to determine FGF-2 surface concentration and retention of FGF-2 binding in vitro. MG-63 cells were uniformly seeded onto patterned substrates. Cells were exposed to defined spatial FGF-2 surface concentrations of 1-22 pg/mm ². Cell numbers were observed to increase in register with the printed FGF-2 patterns from an initial random uniform cell distribution across the patterned and non-patterned regions. Based on time-lapse image analysis, the primary organizational response of the cells was determined to be proliferation and not migration. Cell counts on and off the FGF-2 patterns over time demonstrated an increase in cell density up to a FGF-2 surface concentration of 14 pg/mm². Higher surface concentrations did not result in increased cell density. In addition, the cells on the FGF-2 patterns survived longer than the cells off patterns. Our inkjet printing approach permits the systematic study of cellular responses to defined spatial surface concentrations of immobilized growth factors.

Original language	English (US)
Pages (from-to)	2213-2221
Number of pages	9
Journal	Biomaterials
Volume	27
Issue number	10
DOIs	https://doi.org/10.1016/j.biomaterials.2005.10.021
State	Published - Apr 2006
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported partially by the Office of Naval Research (Grant No. N000140110766), the National Science Foundation (Grants No. CTS-0210238 and DMI-9800565), the National Institutes of Health (Grant No. 1 R01 EB00 364-01), the Pennsylvania Infrastructure Technology Alliance (PITA) from the Pennsylvania Department of Community and Economic Development, the Health Resources and Services Administration (Grant No. 1C76 HF 00381-01), the Scaife Foundation, and the Philip and Marsha Dowd Engineering Seed Fund. We wish to thank Aventis Behring, L.L.C. (King of Prussia, PA) for their generous gift of lyophilized human fibrinogen and thrombin.

Keywords

Biological patterning
Growth factor
Inkjet technology
Printing technology
Solid freeform fabrication
Tissue engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Publisher link

10.1016/j.biomaterials.2005.10.021

Find It

Find It at U of M Libraries

Cite this

@article{0d78b9ac34a04f08a1d3f729f91c2b6b,

title = "Dose-dependent cell growth in response to concentration modulated patterns of FGF-2 printed on fibrin",

abstract = "Immobilized patterns of unmodified fibroblast growth factor-2 (FGF-2), with varying surface concentrations, were inkjet printed onto physiologically relevant fibrin substrates. Printed patterns were characterized using iodinated FGF-2 to determine FGF-2 surface concentration and retention of FGF-2 binding in vitro. MG-63 cells were uniformly seeded onto patterned substrates. Cells were exposed to defined spatial FGF-2 surface concentrations of 1-22 pg/mm 2. Cell numbers were observed to increase in register with the printed FGF-2 patterns from an initial random uniform cell distribution across the patterned and non-patterned regions. Based on time-lapse image analysis, the primary organizational response of the cells was determined to be proliferation and not migration. Cell counts on and off the FGF-2 patterns over time demonstrated an increase in cell density up to a FGF-2 surface concentration of 14 pg/mm2. Higher surface concentrations did not result in increased cell density. In addition, the cells on the FGF-2 patterns survived longer than the cells off patterns. Our inkjet printing approach permits the systematic study of cellular responses to defined spatial surface concentrations of immobilized growth factors.",

keywords = "Biological patterning, Growth factor, Inkjet technology, Printing technology, Solid freeform fabrication, Tissue engineering",

author = "Miller, {Eric D.} and Fisher, {Gregory W.} and Weiss, {Lee E.} and Walker, {Lynn M.} and Campbell, {Phil G.}",

note = "Funding Information: This work was supported partially by the Office of Naval Research (Grant No. N000140110766), the National Science Foundation (Grants No. CTS-0210238 and DMI-9800565), the National Institutes of Health (Grant No. 1 R01 EB00 364-01), the Pennsylvania Infrastructure Technology Alliance (PITA) from the Pennsylvania Department of Community and Economic Development, the Health Resources and Services Administration (Grant No. 1C76 HF 00381-01), the Scaife Foundation, and the Philip and Marsha Dowd Engineering Seed Fund. We wish to thank Aventis Behring, L.L.C. (King of Prussia, PA) for their generous gift of lyophilized human fibrinogen and thrombin. ",

year = "2006",

month = apr,

doi = "10.1016/j.biomaterials.2005.10.021",

language = "English (US)",

volume = "27",

pages = "2213--2221",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier Ltd",

number = "10",

}

TY - JOUR

T1 - Dose-dependent cell growth in response to concentration modulated patterns of FGF-2 printed on fibrin

AU - Miller, Eric D.

AU - Fisher, Gregory W.

AU - Weiss, Lee E.

AU - Walker, Lynn M.

AU - Campbell, Phil G.

N1 - Funding Information: This work was supported partially by the Office of Naval Research (Grant No. N000140110766), the National Science Foundation (Grants No. CTS-0210238 and DMI-9800565), the National Institutes of Health (Grant No. 1 R01 EB00 364-01), the Pennsylvania Infrastructure Technology Alliance (PITA) from the Pennsylvania Department of Community and Economic Development, the Health Resources and Services Administration (Grant No. 1C76 HF 00381-01), the Scaife Foundation, and the Philip and Marsha Dowd Engineering Seed Fund. We wish to thank Aventis Behring, L.L.C. (King of Prussia, PA) for their generous gift of lyophilized human fibrinogen and thrombin.

PY - 2006/4

Y1 - 2006/4

N2 - Immobilized patterns of unmodified fibroblast growth factor-2 (FGF-2), with varying surface concentrations, were inkjet printed onto physiologically relevant fibrin substrates. Printed patterns were characterized using iodinated FGF-2 to determine FGF-2 surface concentration and retention of FGF-2 binding in vitro. MG-63 cells were uniformly seeded onto patterned substrates. Cells were exposed to defined spatial FGF-2 surface concentrations of 1-22 pg/mm 2. Cell numbers were observed to increase in register with the printed FGF-2 patterns from an initial random uniform cell distribution across the patterned and non-patterned regions. Based on time-lapse image analysis, the primary organizational response of the cells was determined to be proliferation and not migration. Cell counts on and off the FGF-2 patterns over time demonstrated an increase in cell density up to a FGF-2 surface concentration of 14 pg/mm2. Higher surface concentrations did not result in increased cell density. In addition, the cells on the FGF-2 patterns survived longer than the cells off patterns. Our inkjet printing approach permits the systematic study of cellular responses to defined spatial surface concentrations of immobilized growth factors.

AB - Immobilized patterns of unmodified fibroblast growth factor-2 (FGF-2), with varying surface concentrations, were inkjet printed onto physiologically relevant fibrin substrates. Printed patterns were characterized using iodinated FGF-2 to determine FGF-2 surface concentration and retention of FGF-2 binding in vitro. MG-63 cells were uniformly seeded onto patterned substrates. Cells were exposed to defined spatial FGF-2 surface concentrations of 1-22 pg/mm 2. Cell numbers were observed to increase in register with the printed FGF-2 patterns from an initial random uniform cell distribution across the patterned and non-patterned regions. Based on time-lapse image analysis, the primary organizational response of the cells was determined to be proliferation and not migration. Cell counts on and off the FGF-2 patterns over time demonstrated an increase in cell density up to a FGF-2 surface concentration of 14 pg/mm2. Higher surface concentrations did not result in increased cell density. In addition, the cells on the FGF-2 patterns survived longer than the cells off patterns. Our inkjet printing approach permits the systematic study of cellular responses to defined spatial surface concentrations of immobilized growth factors.

KW - Biological patterning

KW - Growth factor

KW - Inkjet technology

KW - Printing technology

KW - Solid freeform fabrication

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=28844454581&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28844454581&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2005.10.021

DO - 10.1016/j.biomaterials.2005.10.021

M3 - Article

C2 - 16325254

AN - SCOPUS:28844454581

SN - 0142-9612

VL - 27

SP - 2213

EP - 2221

JO - Biomaterials

JF - Biomaterials

IS - 10

ER -

Dose-dependent cell growth in response to concentration modulated patterns of FGF-2 printed on fibrin

Abstract

Bibliographical note

Keywords

UN SDGs

Publisher link

Find It

Other files and links

Fingerprint

Cite this