DOR undergoes nucleo-cytoplasmic shuttling, which involves passage through the nucleolus

Caroline Mauvezin, Ana Sancho, Saska Ivanova, Manuel Palacin, Antonio Zorzano

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

DOR is a bi-functional protein that regulates transcription and enhances starvation-induced autophagy. While autophagy has been mostly described as a stress-response mechanism, cells also need autophagy to maintain homeostasis in basal conditions. However, the mechanisms regulating basal autophagy still remain unknown. Our results show that DOR acts in basal autophagy. Indeed, DOR already undergoes nucleo-cytoplasmic shuttling in basal conditions and, surprisingly, DOR exits continuously the nucleus and traverses the nucleolus. However, the nucleolus integrity is not essential for both DOR nucleo-cytoplasmic shuttling and DOR function on basal autophagy. Taken together, we propose that DOR exit from the nucleus is essential for basal autophagy stimulation even under nucleolus disruption.

Original languageEnglish (US)
Pages (from-to)3179-3186
Number of pages8
JournalFEBS Letters
Volume586
Issue number19
DOIs
StatePublished - Sep 21 2012

Bibliographical note

Funding Information:
We thank the Advanced Digital Microscopy Facility (IRB Barcelona), in particular Anna Lladó, Lidia Bardia and Carme Casals. Thanks also go to I. Castrillón, and J.C. Monasterio for technical assistance. We thank Ms. T. Yates for editorial support. We thank Dr. Neus Agell for kindly providing plasmids. C.M. and A.S. were respectively a FPI fellow and a FIS fellow (“Instituto de Salud Carlos III)”. This study was supported by research grants from the MEC (SAF2008-03803), Grant 2009SGR915 from the “Generalitat de Catalunya”, CIBERDEM (“Instituto de Salud Carlos III”), (INTERREG IV-B-SUDOE-FEDER (DIOMED, SOE1/P1/E178), and COST Action BM0602. A.Z. was the recipient of a Science Intensification Award from the University of Barcelona.

Keywords

  • Basal autophagy
  • DOR
  • Nucleo-cytoplasmic shuttle
  • Nucleolus
  • TP53inp2

Fingerprint Dive into the research topics of 'DOR undergoes nucleo-cytoplasmic shuttling, which involves passage through the nucleolus'. Together they form a unique fingerprint.

Cite this