Dominant role of N-type Ca2+ channels in evoked release of norepinephrine from sympathetic neurons

Lane D. Hirning, Aaron P. Fox, Edwin W. Mccleskey, Baldomero M. Olivera, Stanley A Thayer, Richard J. Miller, Richard W. Tsien

Research output: Contribution to journalArticlepeer-review

692 Scopus citations


Multiple types of calcium channels have been found in neurons, but uncertainty remains about which ones are involved in stimulus-secretion coupling. Two types of calcium channels in rat sympathetic neurons were described, and their relative importance in controlling norepinephrine release was analyzed. N-type and L-type calcium channels differed in voltage dependence, unitary barium conductance, and pharmacology. Nitrendipine inhibited activity of L-type channels but not N-type channels. Potassium-evoked norepinephrine release was markedly reduced by cadmium and the conesnail peptide toxin ω-Conus geographus toxin VIA, agents that block both N- and L-type channels, but was little affected by nitrendipine at concentrations that strongly reduce calcium influx, as measured by fura-2. Thus N-type calcium channels play a dominant role in the depolarization-evoked release of norepinephrine.

Original languageEnglish (US)
Pages (from-to)57-61
Number of pages5
Issue number4835
StatePublished - 1988
Externally publishedYes


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