Abstract
Prior work resulted in identification of an Arabidopsis mutant, eer5-1, with extreme ethylene response in conjunction with failure to induce a subset of ethylene-responsive genes, including AtEBP. EER5, which is a TREX-2 homolog that is part of a nucleoporin complex, functions as part of a cryptic aspect of the ethylene signaling pathway that is required for regulating the magnitude of ethylene response. A suppressor mutagenesis screen was carried out to identify second site mutations that could restore the growth of ethylene-treated eer5-1 to wild-type levels. A dominant gain-of-function mutation in the ethylene receptor ETHYLENE RESPONSE SENSOR 1 (ERS1) was identified, with the ers1-4 mutation being located in transmembrane domain III at a point nearly equivalent to the previously described etr1-2 mutation in the other Arabidopsis subfamily I ethylene receptor, ETHYLENE RESPONSE 1 (ETR1). Although both ers1-4 and etr1-2 partially suppress the ethylene hypersensitivity of eer5-1 and are at least in part REVERSION TO ETHYLENE SENSITIVITY 1 (RTE1)-dependent, ers1-4 was additionally found to restore the expression of AtEBP in ers1-4;eer5-1 etiolated seedlings after ethylene treatment in an EIN3-dependent manner. Our work indicates that ERS1-regulated expression of a subset of ethylene-responsive genes is related to controlling the magnitude of ethylene response, with hyperinduction of these genes correlated with reduced ethylene-dependent growth inhibition.
Original language | English (US) |
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Pages (from-to) | 442-455 |
Number of pages | 14 |
Journal | New Phytologist |
Volume | 208 |
Issue number | 2 |
DOIs | |
State | Published - Oct 1 2015 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2015 New Phytologist Trust.
Keywords
- Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP)
- EER5
- EIN3
- ETHYLENE RESPONSE 1 (ETR1)
- ETHYLENE RESPONSE SENSOR 1 (ERS1)
- Ethylene
- REVERSION TO ETHYLENE SENSITIVITY 1 (RTE1)