Domains in the 1α dynein heavy chain required for inner arm assembly and flagellar motility in Chlamydomonas

Steven H. Myster, Julie A. Knott, Katrina M. Wysocki, Eileen O'Toole, Mary E Porter

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Flagellar motility is generated by the activity of multiple dynein motors, but the specific role of each dynein heavy chain (Dhc) is largely unknown, and the mechanism by which the different Dhcs are targeted to their unique locations is also poorly understood. We report here the complete nucleotide sequence of the Chlamydomonas Dhc1 gene and the corresponding deduced amino acid sequence of the 1α Dhc of the I1 inner dynein arm. The 1α Dhc is similar to other axomal Dhcs, but two additional phosphate binding motifs (P-loops) have been identified in the NH2- and COOH-terminal regions. Because mutations in Dhc1 result in motility defects and loss of the I1 inner arm, a series of Dhc1 transgenes were used to rescue the mutant phenotypes. Motile cotransformants that express either full-length or truncated 1α Dhcs were recovered. The truncated 1α Dhc fragments lacked the dynein motor domain, but still assembled with the 1β Dhc and other I1 subunits into partially functional complexes at the correct axoneme location. Analysis of the transformants has identified the site of the 1α motor domain in the I1 structure and further revealed the role of the 1α Dhc in flagellar motility and phototactic behavior.

Original languageEnglish (US)
Pages (from-to)801-818
Number of pages18
JournalJournal of Cell Biology
Volume146
Issue number4
DOIs
StatePublished - Aug 23 1999

Fingerprint

Chlamydomonas
Dyneins
Axoneme
Transgenes
Amino Acid Sequence
Phosphates
Phenotype
Mutation

Keywords

  • Dynein
  • Flagella
  • Inner arm
  • Motors
  • Phototaxis

Cite this

Domains in the 1α dynein heavy chain required for inner arm assembly and flagellar motility in Chlamydomonas. / Myster, Steven H.; Knott, Julie A.; Wysocki, Katrina M.; O'Toole, Eileen; Porter, Mary E.

In: Journal of Cell Biology, Vol. 146, No. 4, 23.08.1999, p. 801-818.

Research output: Contribution to journalArticle

@article{0af1ddd1a4524c168b998b03075df944,
title = "Domains in the 1α dynein heavy chain required for inner arm assembly and flagellar motility in Chlamydomonas",
abstract = "Flagellar motility is generated by the activity of multiple dynein motors, but the specific role of each dynein heavy chain (Dhc) is largely unknown, and the mechanism by which the different Dhcs are targeted to their unique locations is also poorly understood. We report here the complete nucleotide sequence of the Chlamydomonas Dhc1 gene and the corresponding deduced amino acid sequence of the 1α Dhc of the I1 inner dynein arm. The 1α Dhc is similar to other axomal Dhcs, but two additional phosphate binding motifs (P-loops) have been identified in the NH2- and COOH-terminal regions. Because mutations in Dhc1 result in motility defects and loss of the I1 inner arm, a series of Dhc1 transgenes were used to rescue the mutant phenotypes. Motile cotransformants that express either full-length or truncated 1α Dhcs were recovered. The truncated 1α Dhc fragments lacked the dynein motor domain, but still assembled with the 1β Dhc and other I1 subunits into partially functional complexes at the correct axoneme location. Analysis of the transformants has identified the site of the 1α motor domain in the I1 structure and further revealed the role of the 1α Dhc in flagellar motility and phototactic behavior.",
keywords = "Dynein, Flagella, Inner arm, Motors, Phototaxis",
author = "Myster, {Steven H.} and Knott, {Julie A.} and Wysocki, {Katrina M.} and Eileen O'Toole and Porter, {Mary E}",
year = "1999",
month = "8",
day = "23",
doi = "10.1083/jcb.146.4.801",
language = "English (US)",
volume = "146",
pages = "801--818",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "4",

}

TY - JOUR

T1 - Domains in the 1α dynein heavy chain required for inner arm assembly and flagellar motility in Chlamydomonas

AU - Myster, Steven H.

AU - Knott, Julie A.

AU - Wysocki, Katrina M.

AU - O'Toole, Eileen

AU - Porter, Mary E

PY - 1999/8/23

Y1 - 1999/8/23

N2 - Flagellar motility is generated by the activity of multiple dynein motors, but the specific role of each dynein heavy chain (Dhc) is largely unknown, and the mechanism by which the different Dhcs are targeted to their unique locations is also poorly understood. We report here the complete nucleotide sequence of the Chlamydomonas Dhc1 gene and the corresponding deduced amino acid sequence of the 1α Dhc of the I1 inner dynein arm. The 1α Dhc is similar to other axomal Dhcs, but two additional phosphate binding motifs (P-loops) have been identified in the NH2- and COOH-terminal regions. Because mutations in Dhc1 result in motility defects and loss of the I1 inner arm, a series of Dhc1 transgenes were used to rescue the mutant phenotypes. Motile cotransformants that express either full-length or truncated 1α Dhcs were recovered. The truncated 1α Dhc fragments lacked the dynein motor domain, but still assembled with the 1β Dhc and other I1 subunits into partially functional complexes at the correct axoneme location. Analysis of the transformants has identified the site of the 1α motor domain in the I1 structure and further revealed the role of the 1α Dhc in flagellar motility and phototactic behavior.

AB - Flagellar motility is generated by the activity of multiple dynein motors, but the specific role of each dynein heavy chain (Dhc) is largely unknown, and the mechanism by which the different Dhcs are targeted to their unique locations is also poorly understood. We report here the complete nucleotide sequence of the Chlamydomonas Dhc1 gene and the corresponding deduced amino acid sequence of the 1α Dhc of the I1 inner dynein arm. The 1α Dhc is similar to other axomal Dhcs, but two additional phosphate binding motifs (P-loops) have been identified in the NH2- and COOH-terminal regions. Because mutations in Dhc1 result in motility defects and loss of the I1 inner arm, a series of Dhc1 transgenes were used to rescue the mutant phenotypes. Motile cotransformants that express either full-length or truncated 1α Dhcs were recovered. The truncated 1α Dhc fragments lacked the dynein motor domain, but still assembled with the 1β Dhc and other I1 subunits into partially functional complexes at the correct axoneme location. Analysis of the transformants has identified the site of the 1α motor domain in the I1 structure and further revealed the role of the 1α Dhc in flagellar motility and phototactic behavior.

KW - Dynein

KW - Flagella

KW - Inner arm

KW - Motors

KW - Phototaxis

UR - http://www.scopus.com/inward/record.url?scp=0033598021&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033598021&partnerID=8YFLogxK

U2 - 10.1083/jcb.146.4.801

DO - 10.1083/jcb.146.4.801

M3 - Article

C2 - 10459015

AN - SCOPUS:0033598021

VL - 146

SP - 801

EP - 818

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 4

ER -