Domain Organization and Active Site Architecture of a Polyketide Synthase C-methyltransferase

Meredith A. Skiba, Andrew P. Sikkema, William D. Fiers, William H. Gerwick, David H. Sherman, Courtney C. Aldrich, Janet L. Smith

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Polyketide metabolites produced by modular type I polyketide synthases (PKS) acquire their chemical diversity through the variety of catalytic domains within modules of the pathway. Methyltransferases are among the least characterized of the catalytic domains common to PKS systems. We determined the domain boundaries and characterized the activity of a PKS C-methyltransferase (C-MT) from the curacin A biosynthetic pathway. The C-MT catalyzes S-adenosylmethionine-dependent methyl transfer to the α-position of β-ketoacyl substrates linked to acyl carrier protein (ACP) or a small-molecule analog but does not act on β-hydroxyacyl substrates or malonyl-ACP. Key catalytic residues conserved in both bacterial and fungal PKS C-MTs were identified in a 2 Å crystal structure and validated biochemically. Analysis of the structure and the sequences bordering the C-MT provides insight into the positioning of this domain within complete PKS modules.

Original languageEnglish (US)
Pages (from-to)3319-3327
Number of pages9
JournalACS Chemical Biology
Volume11
Issue number12
DOIs
StatePublished - Dec 16 2016

Bibliographical note

Funding Information:
This work was supported by National Institutes of Health grants DK042303 to J.L.S.; CA108874 to D.H.S., W.H.G., and J.L.S.; and GM118101 to D.H.S. M.A.S. was supported by a predoctoral fellowship from the Cellular Biotechnology Training Program (T32GM008353) and Rackham Graduate School. GM/CA@APS is supported by the National Institutes of Health National Institute of General Medical Sciences (AGM-12006) and National Cancer Institute (ACB-12002). The Advanced Photon Source is a U.S. Department of Energy (DOE) Office of Science User Facility operated by Argonne National Laboratory under Contract No. DE-AC02-06CH11357.

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