Does pneumoperitoneum during laparoscopy increase bacterial translocation?

M. R. Evasovich, T. C. Clark, M. C. Horattas, S. Holda, L. Treen

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

Background: To evaluate the impact of laparoscopy in the presence of peritonitis, this study was designed to assess bacteremia caused by E. coli-induced peritonitis with a carbon dioxide pneumoperitoneum in a rat model. Methods: Sixty Sprague-Dawley rats were divided into inoculum groups (no E. coli, 106 colony-forming units [CFU] E. coli, and 108 CFU E. coli), followed by induction of a carbon dioxide pneumoperitoneum or no pneumoperitoneum. Fifteen-minute-interval blood cultures were obtained to determine time of bacteremia development. Statistical assessment to determine significant differences among groups was done using ANOVA and t-test analysis. Results: A total of 20 animals with E. coli introduced into the peritoneum and a carbon-dioxide-induced pneumoperitoneum had more frequent positive blood cultures at all time intervals compared to identical inoculum subgroups without a pneumoperitoneum. ANOVA revealed a significant difference in bacteremia within the same concentration inoculum groups in animals receiving a pneumoperitoneum vs none (p < 0.01). Bacteremia increased significantly as inoculum concentrations increased (25% with 106 E. coli inoculum vs 80% with 108 E. coli), especially among the insufflated subgroups (45% with 106 E. coli vs 100% with 108 E. coli) over 180 min (p < 0.01). Conclusion: Carbon dioxide pneumoperitoneum increases the incidence of E. coli bacterial translocation from the peritoneum into the bloodstream in this rat model.

Original languageEnglish (US)
Pages (from-to)1176-1179
Number of pages4
JournalSurgical endoscopy
Volume10
Issue number12
DOIs
StatePublished - Dec 1996

Keywords

  • Bacteremia
  • Blood cultures
  • Carbon dioxide
  • Peritonitis
  • Pneumoperitoneum

Fingerprint

Dive into the research topics of 'Does pneumoperitoneum during laparoscopy increase bacterial translocation?'. Together they form a unique fingerprint.

Cite this