DNA topoisomerases as targets for antibacterial agents

Research output: Chapter in Book/Report/Conference proceedingChapter

16 Scopus citations

Abstract

DNA topoisomerases are proven therapeutic targets of antibacterial agents. Quinolones, especially fluoroquinolones, are the most successful topoisomerase-targeting antibacterial drugs. These drugs target type IIA topoisomerases in bacteria. Recent structural and biochemical studies on fluoroquinolones have provided the molecular basis for both their mechanism of action, as well as the molecular basis of bacterial resistance. Due to the development of drug resistance, including fluoroquinolone resistance, among bacterial pathogens, there is an urgent need to discover novel antibacterial agents. Recent advances in topoisomerase inhibitors may lead to the development of novel antibacterial drugs that are effective against fluoroquinolone-resistant pathogens. They include type IIA topoisomerase inhibitors that either interact with the GyrB/ParE subunit or form nick-containing ternary complexes. In addition, several topoisomerase I inhibitors have recently been identified. Thus, DNA topoisomerases remain important targets of antibacterial agents.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages47-62
Number of pages16
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1703
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
I would like to thank Lisa Oppegard for her contribution and critical comments on the manuscript, Fang Li for invaluable discussion, and Justine Delgado for preparation of Fig. 1 and critical comments on the manuscript. Studies from my laboratory were supported in part by National Institutes of Health grants GM59465, AI087671, and a fellowship from SmithKline Beecham Pharmaceuticals.

Keywords

  • Aminocoumarin
  • Antibacterial drugs
  • DNA gyrase
  • Fluoroquinolone
  • Topoisomerase I
  • Topoisomerase IV
  • Topoisomerase poison

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