DNA-templated assembly of droplet-derived PEG microtissues

Cheri Y. Li, David K. Wood, Caroline M. Hsu, Sangeeta N. Bhatia

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Patterning multiple cell types is a critical step for engineering functional tissues, but few methods provide three-dimensional positioning at the cellular length scale. Here, we present a "bottom-up" approach for fabricating multicellular tissue constructs that utilizes DNA-templated assembly of 3D cell-laden hydrogel microtissues. A flow focusing-generated emulsion of photopolymerizable prepolymer is used to produce 100 μm monodisperse microtissues at a rate of 100 Hz (105 h-1). Multiple cell types, including suspension and adherently cultured cells, can be encapsulated into the microtissues with high viability (∼97%). We then use a DNA coding scheme to self-assemble microtissues "bottom-up" from a template that is defined using "top-down" techniques. The microtissues are derivatized with single-stranded DNA using a biotin-streptavidin linkage to the polymer network, and are assembled by sequence-specific hybridization onto spotted DNA microarrays. Using orthogonal DNA codes, we achieve multiplexed patterning of multiple microtissue types with high binding efficiency and >90% patterning specificity. Finally, we demonstrate the ability to organize multicomponent constructs composed of epithelial and mesenchymal microtissues while preserving each cell type in a 3D microenvironment. The combination of high throughput microtissue generation with scalable surface-templated assembly offers the potential to dissect mechanisms of cell-cell interaction in three dimensions in healthy and diseased states, as well as provides a framework for templated assembly of larger structures for implantation.

Original languageEnglish (US)
Pages (from-to)2967-2975
Number of pages9
JournalLab on a chip
Volume11
Issue number17
DOIs
StatePublished - Sep 7 2011

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