DNA synthesis associated with a DNA-nuclear-membrane complex from rat liver

Akhouri A Sinha, Nobuko S. Mizuno

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Ultrastructural analysis of M-band from nuclei of rat liver showed small amounts of chromatin, fragments of inner nuclear membrane, some amorphous nuclear material, and nucleopores. The outer nuclear membrane with its associated ribosomes was removed by Sarkosyl during the preparation of the M-band sample. Morphological features of nucleopores and the inner nuclear membrane were confirmed by freeze-fracture technique. The gross chemical composition of the M-band was similar to that of nuclear membrane fractions prepared by other techniques. The M-band contained the greatest proportion of newly-labeled DNA and also supported DNA synthesis in vitro. Electron-microscopic autoradiography of the M-band showed localization of silver grains of thymidine-3H presumably over newly synthesized DNA. The DNA synthesis could not be attributed to spurious attachment of DNA polymerase to M-band during its isolation. It was partially removed from the M-band by treatment with 0.5 M KC1, phospholipase A or C; and completely, by the action of pancreatic DNase. DNA synthesis was greater in M-band fractions isolated from nuclei of 24-hour regenerating liver.

Original languageEnglish (US)
Pages (from-to)191-201
Number of pages11
JournalCell and Tissue Research
Volume183
Issue number2
DOIs
StatePublished - Sep 1 1977

Keywords

  • DNA
  • DNA synthesis
  • M-band Nucleopores
  • Nuclear membrane complex
  • Rat Liver

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