Dna mobility in crystals of a nonspecific λ, croldna complex

Dmitry E. Klimenko, Boris K. Chernov, Lucy V. Malinina

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

DNA location in the crystal of the nonspecific λ cro/(GT)4*(AC)4complex has been studied by the isomorphous replacement method using iodinated and brominated oligonucleotides. The results of the search for heavy atom positions combined with previously obtained molecular replacement data suggest that the DNA octamer occupies two overlapping positions, each of the two duplexes (GTGTGTGT)*(ACACACAC) belonging to the same imaginary longer double helix and differing only in the shift by two base pairs along the common sugar-phosphate backbone. In the crystals of the heavy atom derivatives different orientations of the DNA octamer are observed as well. It seems reasonable that the DNA mobility of both kinds might be a common feature of crystals of nonspecific repressor/DNA complexes.

Original languageEnglish (US)
Pages (from-to)529-536
Number of pages8
JournalJournal of Biomolecular Structure and Dynamics
Volume13
Issue number3
DOIs
StatePublished - Dec 1995

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