DNA methylation in a sea lamprey vasotocin receptor gene promoter correlates with tissue- and life-stage-specific mRNA expression

Sally A. Mayasich, Lynne T Bemis, Benjamin L Clarke

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The jawless vertebrate sea lamprey (Petromyzon marinus) genome has a different structure from both invertebrates and jawed vertebrates featuring high guanine–cytosine (GC) content. This raises the question of whether DNA methylation of cytosine–guanine (CpG) dinucleotides could function to regulate lamprey gene transcription. We previously characterized a lamprey arginine vasotocin (AVT) receptor gene (Pm807) possessing characteristics of both arginine vasopressin (AVP) V1A and oxytocin (OXT) receptor genes of jawed vertebrates. Lamprey Pm807 mRNA is highly expressed in adult heart and larval liver but not expressed in adult liver. Using high-resolution melt (HRM) PCR on bisulfite-converted DNA, we pinpointed a region with tissue-specific differences in DNA melt characteristics, indicating differences in methylation level. Sequencing revealed a pattern of methylation at specific CpGs at consistently higher levels in adult heart and larval liver than adult liver. These CpGs are associated with putative transcription factor binding sequences organized similarly to functional OXTR promoters in mammals, suggesting functional similarity in lamprey gene transcription regulation.

Original languageEnglish (US)
Pages (from-to)56-66
Number of pages11
JournalComparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
Volume202
DOIs
StatePublished - Dec 1 2016

Fingerprint

Petromyzon
Lampreys
DNA Methylation
Genes
Liver
Tissue
Vertebrates
Messenger RNA
Methylation
Transcription
Oxytocin Receptors
Vasotocin
Vasopressin Receptors
DNA
Invertebrates
Mammals
Transcription Factors
Genome
Polymerase Chain Reaction
vasotocin receptor

Keywords

  • DNA methylation
  • Estrogen response element
  • Oxytocin receptor
  • Sea lamprey
  • Vasotocin receptor
  • Zinc-finger transcription factors

Cite this

@article{5d24585e6c1f4101a174b07a2fc0411f,
title = "DNA methylation in a sea lamprey vasotocin receptor gene promoter correlates with tissue- and life-stage-specific mRNA expression",
abstract = "The jawless vertebrate sea lamprey (Petromyzon marinus) genome has a different structure from both invertebrates and jawed vertebrates featuring high guanine–cytosine (GC) content. This raises the question of whether DNA methylation of cytosine–guanine (CpG) dinucleotides could function to regulate lamprey gene transcription. We previously characterized a lamprey arginine vasotocin (AVT) receptor gene (Pm807) possessing characteristics of both arginine vasopressin (AVP) V1A and oxytocin (OXT) receptor genes of jawed vertebrates. Lamprey Pm807 mRNA is highly expressed in adult heart and larval liver but not expressed in adult liver. Using high-resolution melt (HRM) PCR on bisulfite-converted DNA, we pinpointed a region with tissue-specific differences in DNA melt characteristics, indicating differences in methylation level. Sequencing revealed a pattern of methylation at specific CpGs at consistently higher levels in adult heart and larval liver than adult liver. These CpGs are associated with putative transcription factor binding sequences organized similarly to functional OXTR promoters in mammals, suggesting functional similarity in lamprey gene transcription regulation.",
keywords = "DNA methylation, Estrogen response element, Oxytocin receptor, Sea lamprey, Vasotocin receptor, Zinc-finger transcription factors",
author = "Mayasich, {Sally A.} and Bemis, {Lynne T} and Clarke, {Benjamin L}",
year = "2016",
month = "12",
day = "1",
doi = "10.1016/j.cbpb.2016.07.007",
language = "English (US)",
volume = "202",
pages = "56--66",
journal = "Comparative biochemistry and physiology. B, Comparative biochemistry",
issn = "1096-4959",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - DNA methylation in a sea lamprey vasotocin receptor gene promoter correlates with tissue- and life-stage-specific mRNA expression

AU - Mayasich, Sally A.

AU - Bemis, Lynne T

AU - Clarke, Benjamin L

PY - 2016/12/1

Y1 - 2016/12/1

N2 - The jawless vertebrate sea lamprey (Petromyzon marinus) genome has a different structure from both invertebrates and jawed vertebrates featuring high guanine–cytosine (GC) content. This raises the question of whether DNA methylation of cytosine–guanine (CpG) dinucleotides could function to regulate lamprey gene transcription. We previously characterized a lamprey arginine vasotocin (AVT) receptor gene (Pm807) possessing characteristics of both arginine vasopressin (AVP) V1A and oxytocin (OXT) receptor genes of jawed vertebrates. Lamprey Pm807 mRNA is highly expressed in adult heart and larval liver but not expressed in adult liver. Using high-resolution melt (HRM) PCR on bisulfite-converted DNA, we pinpointed a region with tissue-specific differences in DNA melt characteristics, indicating differences in methylation level. Sequencing revealed a pattern of methylation at specific CpGs at consistently higher levels in adult heart and larval liver than adult liver. These CpGs are associated with putative transcription factor binding sequences organized similarly to functional OXTR promoters in mammals, suggesting functional similarity in lamprey gene transcription regulation.

AB - The jawless vertebrate sea lamprey (Petromyzon marinus) genome has a different structure from both invertebrates and jawed vertebrates featuring high guanine–cytosine (GC) content. This raises the question of whether DNA methylation of cytosine–guanine (CpG) dinucleotides could function to regulate lamprey gene transcription. We previously characterized a lamprey arginine vasotocin (AVT) receptor gene (Pm807) possessing characteristics of both arginine vasopressin (AVP) V1A and oxytocin (OXT) receptor genes of jawed vertebrates. Lamprey Pm807 mRNA is highly expressed in adult heart and larval liver but not expressed in adult liver. Using high-resolution melt (HRM) PCR on bisulfite-converted DNA, we pinpointed a region with tissue-specific differences in DNA melt characteristics, indicating differences in methylation level. Sequencing revealed a pattern of methylation at specific CpGs at consistently higher levels in adult heart and larval liver than adult liver. These CpGs are associated with putative transcription factor binding sequences organized similarly to functional OXTR promoters in mammals, suggesting functional similarity in lamprey gene transcription regulation.

KW - DNA methylation

KW - Estrogen response element

KW - Oxytocin receptor

KW - Sea lamprey

KW - Vasotocin receptor

KW - Zinc-finger transcription factors

UR - http://www.scopus.com/inward/record.url?scp=84983428114&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84983428114&partnerID=8YFLogxK

U2 - 10.1016/j.cbpb.2016.07.007

DO - 10.1016/j.cbpb.2016.07.007

M3 - Article

C2 - 27497665

AN - SCOPUS:84983428114

VL - 202

SP - 56

EP - 66

JO - Comparative biochemistry and physiology. B, Comparative biochemistry

JF - Comparative biochemistry and physiology. B, Comparative biochemistry

SN - 1096-4959

ER -