DNA dissociation and degradation at gold nanoparticle surfaces

Aimee R. Herdt; Sarah M Drawz; Youngjong Kang; T. A Taton

doi:10.1016/j.colsurfb.2006.06.006

DNA dissociation and degradation at gold nanoparticle surfaces

Aimee R. Herdt, Sarah M Drawz, Youngjong Kang, T. A Taton

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

The instability of DNA-functionalized gold nanoparticles (Au-DNA conjugates) upon exposure to high temperatures is characterized using fluorescence spectroscopy, gel electrophoresis and ion-exchange chromatography. Above 70 °C, aqueous Au-DNA conjugates decompose within hours due to both desorption of thiol-terminated DNA from the gold nanoparticle surface and chemical degradation of DNA in the presence of colloidal gold. Although the chemical mechanism for DNA degradation was not identified in this study, the gold surface participates directly in the cleavage reaction. These results have important implications for the use of Au-DNA conjugates in biotechnological and clinical applications that require high temperatures, such as polymerase chain reaction (PCR).

Original language	English (US)
Pages (from-to)	130-139
Number of pages	10
Journal	Colloids and Surfaces B: Biointerfaces
Volume	51
Issue number	2
DOIs	https://doi.org/10.1016/j.colsurfb.2006.06.006
State	Published - Aug 15 2006

Bibliographical note

Funding Information:
We thank the NIH Chemical–Biology Interface Training Grant for graduate student support of this work and the University of Minnesota.

Keywords

Au-DNA nanoparticle conjugates
DNA degradation
Thiol desorption

Publisher link

10.1016/j.colsurfb.2006.06.006

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title = "DNA dissociation and degradation at gold nanoparticle surfaces",

abstract = "The instability of DNA-functionalized gold nanoparticles (Au-DNA conjugates) upon exposure to high temperatures is characterized using fluorescence spectroscopy, gel electrophoresis and ion-exchange chromatography. Above 70 °C, aqueous Au-DNA conjugates decompose within hours due to both desorption of thiol-terminated DNA from the gold nanoparticle surface and chemical degradation of DNA in the presence of colloidal gold. Although the chemical mechanism for DNA degradation was not identified in this study, the gold surface participates directly in the cleavage reaction. These results have important implications for the use of Au-DNA conjugates in biotechnological and clinical applications that require high temperatures, such as polymerase chain reaction (PCR).",

keywords = "Au-DNA nanoparticle conjugates, DNA degradation, Thiol desorption",

author = "Herdt, {Aimee R.} and Drawz, {Sarah M} and Youngjong Kang and Taton, {T. A}",

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T1 - DNA dissociation and degradation at gold nanoparticle surfaces

AU - Herdt, Aimee R.

AU - Drawz, Sarah M

AU - Kang, Youngjong

AU - Taton, T. A

N1 - Funding Information: We thank the NIH Chemical–Biology Interface Training Grant for graduate student support of this work and the University of Minnesota.

PY - 2006/8/15

Y1 - 2006/8/15

N2 - The instability of DNA-functionalized gold nanoparticles (Au-DNA conjugates) upon exposure to high temperatures is characterized using fluorescence spectroscopy, gel electrophoresis and ion-exchange chromatography. Above 70 °C, aqueous Au-DNA conjugates decompose within hours due to both desorption of thiol-terminated DNA from the gold nanoparticle surface and chemical degradation of DNA in the presence of colloidal gold. Although the chemical mechanism for DNA degradation was not identified in this study, the gold surface participates directly in the cleavage reaction. These results have important implications for the use of Au-DNA conjugates in biotechnological and clinical applications that require high temperatures, such as polymerase chain reaction (PCR).

AB - The instability of DNA-functionalized gold nanoparticles (Au-DNA conjugates) upon exposure to high temperatures is characterized using fluorescence spectroscopy, gel electrophoresis and ion-exchange chromatography. Above 70 °C, aqueous Au-DNA conjugates decompose within hours due to both desorption of thiol-terminated DNA from the gold nanoparticle surface and chemical degradation of DNA in the presence of colloidal gold. Although the chemical mechanism for DNA degradation was not identified in this study, the gold surface participates directly in the cleavage reaction. These results have important implications for the use of Au-DNA conjugates in biotechnological and clinical applications that require high temperatures, such as polymerase chain reaction (PCR).

KW - Au-DNA nanoparticle conjugates

KW - DNA degradation

KW - Thiol desorption

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DNA dissociation and degradation at gold nanoparticle surfaces

Abstract

Bibliographical note

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