DNA cleavage system of nanosized graphene oxide sheets and copper ions

Hongliu Ren, Chong Wang, Jiali Zhang, Xuejiao Zhou, Dafeng Xu, Jing Zheng, Shouwu Guo, Jingyan Zhang

Research output: Contribution to journalArticlepeer-review

102 Scopus citations

Abstract

The exploration of efficient DNA intercalative agents (intercalators) is essential for understanding DNA scission, repair, and signal transduction. In this work, we explored systematically the graphene oxide (GO) interaction with DNA molecules using fluorescence spectroscopic (FL) and circular dichroism (CD) studies, gel electrophoresis, and DNA thermal denaturation. We demonstrated that the GO nanosheets could intercalate efficiently into DNA molecules. Significantly, we illustrated that the scission of DNA by GO sheets combining with copper ions could take place pronouncedly. The scission of DNA by the GO/Cu2+ system is critically dependent on the concentrations of GO and Cu2+ and their ratio. DNA cleavage ability exhibited by the GO with several other metal ions and the fact that GO/Cu2+-cleaved DNA fragments can be partially relegated suggest that the mechanism of DNA cleavage by the GO/metal ion system is oxidative and hydrolytic. The result reveals that the GO/Cu2+ could be used as a DNA cleaving system that should find many practical applications in biotechnology and as therapeutic agents.

Original languageEnglish (US)
Pages (from-to)7169-7174
Number of pages6
JournalACS nano
Volume4
Issue number12
DOIs
StatePublished - Dec 28 2010
Externally publishedYes

Keywords

  • DNA cleavage
  • DNA intercalation
  • copper ion
  • graphene oxide
  • metal ions

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