DNA between variable and joining gene segments of immunoglobulin kappa light chain is frequently retained in cells that rearrange the kappa locus

B. G. Van Ness, C. Coleclough, R. P. Perry, M. Weigert

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

A systematic analysis of the fate of the DNA between κ chain variable (V(κ)) and joining (J(κ)) genes in cells that have rearranged κ loci was carried out. The DNA from a variety of κ plasmacytomas, λ-producing hybridomas, and κ lymphocytes was digested, fractionated by size, and analyzed with two probes containing sequences 5' of J(κ). In 13 of 28 plasmacytomas examined the rearrangement of V(κ) and J(κ) appears to be acompanied by loss of DNA upstream of J(κ). However, in the rest of the plasmacytomas one or more upstream sequences are retained in a new context. In 9 of 12 λ-producing hybridomas (which frequently rearrange both κ loci) one or more upstream segments were detected. These unique fragments were probably generated by a recombination event near or at the J(κ) region. The extent to which the region between V and J is maintained in κ-expressing lymphocytes was also measured. Most (76%) of the region upstream of J(κ) is retained in the population, even though 68% of the κ loci are rearranged. In order to explain how these up-stream elements occur in some, but not all, cell lines, and the significant occurrence in the lymphocyte population, we propose a model in which a step in V-J joining involves mitotic recombination by unequal sister chromatid exchange.

Original languageEnglish (US)
Pages (from-to)262-266
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume79
Issue number2 I
DOIs
StatePublished - Jan 1 1982

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