DNA arms do the legwork to ensure the directionality of λ site-specific recombination

Marta Radman-Livaja, Tapan Biswas, Tom Ellenberger, Arthur Landy, Hideki Aihara

Research output: Contribution to journalReview article

46 Citations (Scopus)

Abstract

The integrase protein of bacteriophage λ (Int) catalyzes site-specific recombination between λ phage and Escherichia coli genomes. Int is a tyrosine recombinase that binds to DNA core sites via a C-terminal catalytic domain and to a collection of arm DNA sites, distant from the site of recombination, via its N-terminal domain. The arm sites, in conjunction with accessory DNA-bending proteins, provide a means of regulating the efficiency and directionality of Int-catalyzed recombination. Recent crystal structures of λ Int tetramers bound to synaptic and Holliday junction intermediates, together with new biochemical data, suggest a mechanism for the allosteric control of the recombination reaction through arm DNA binding interactions.

Original languageEnglish (US)
Pages (from-to)42-50
Number of pages9
JournalCurrent Opinion in Structural Biology
Volume16
Issue number1
DOIs
StatePublished - Feb 1 2006

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Genetic Recombination
DNA
Cruciform DNA
Coliphages
Integrases
Recombinases
Bacteriophages
Tyrosine
Catalytic Domain
Proteins
Genome

Cite this

DNA arms do the legwork to ensure the directionality of λ site-specific recombination. / Radman-Livaja, Marta; Biswas, Tapan; Ellenberger, Tom; Landy, Arthur; Aihara, Hideki.

In: Current Opinion in Structural Biology, Vol. 16, No. 1, 01.02.2006, p. 42-50.

Research output: Contribution to journalReview article

Radman-Livaja, Marta ; Biswas, Tapan ; Ellenberger, Tom ; Landy, Arthur ; Aihara, Hideki. / DNA arms do the legwork to ensure the directionality of λ site-specific recombination. In: Current Opinion in Structural Biology. 2006 ; Vol. 16, No. 1. pp. 42-50.
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