DNA aptamers targeting P. aeruginosa RNAP

Hari Prasad Timilsina; Dmytro Kompaniiets; Satya Arya; Rick Postema; Raunak Jahan; Andrew Michael Reynolds; Siddhartha Thennakoon; Bin Liu; Xiaohong Tan

doi:10.1039/d5cc00682a

DNA aptamers targeting P. aeruginosa RNAP

Hari Prasad Timilsina, Dmytro Kompaniiets, Satya Arya, Rick Postema, Raunak Jahan, Andrew Michael Reynolds, Siddhartha Thennakoon, Bin Liu, Xiaohong Tan

The Hormel Institute

Research output: Contribution to journal › Article › peer-review

Abstract

We present the first DNA aptamers designed to target the RNA polymerase (RNAP) of Pseudomonas aeruginosa. Utilizing SELEX, we identified and examined aptamers, among which the R2 aptamer demonstrated high specificity and significant binding affinity for RNAP. R2 effectively captured RNAP, making it suitable for protein tandom purification and coating applications. These results have revealed that aptamers are valuable tools for investigating P. aeruginosa RNAP.

Original language	English (US)
Pages (from-to)	4848-4851
Number of pages	4
Journal	Chemical Communications
Volume	61
Issue number	25
DOIs	https://doi.org/10.1039/d5cc00682a
State	Published - Feb 28 2025

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© 2025 The Royal Society of Chemistry.

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title = "DNA aptamers targeting P. aeruginosa RNAP",

abstract = "We present the first DNA aptamers designed to target the RNA polymerase (RNAP) of Pseudomonas aeruginosa. Utilizing SELEX, we identified and examined aptamers, among which the R2 aptamer demonstrated high specificity and significant binding affinity for RNAP. R2 effectively captured RNAP, making it suitable for protein tandom purification and coating applications. These results have revealed that aptamers are valuable tools for investigating P. aeruginosa RNAP.",

author = "Timilsina, {Hari Prasad} and Dmytro Kompaniiets and Satya Arya and Rick Postema and Raunak Jahan and Reynolds, {Andrew Michael} and Siddhartha Thennakoon and Bin Liu and Xiaohong Tan",

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doi = "10.1039/d5cc00682a",

language = "English (US)",

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AU - Timilsina, Hari Prasad

AU - Kompaniiets, Dmytro

AU - Arya, Satya

AU - Postema, Rick

AU - Jahan, Raunak

AU - Reynolds, Andrew Michael

AU - Thennakoon, Siddhartha

AU - Liu, Bin

AU - Tan, Xiaohong

PY - 2025/2/28

Y1 - 2025/2/28

N2 - We present the first DNA aptamers designed to target the RNA polymerase (RNAP) of Pseudomonas aeruginosa. Utilizing SELEX, we identified and examined aptamers, among which the R2 aptamer demonstrated high specificity and significant binding affinity for RNAP. R2 effectively captured RNAP, making it suitable for protein tandom purification and coating applications. These results have revealed that aptamers are valuable tools for investigating P. aeruginosa RNAP.

AB - We present the first DNA aptamers designed to target the RNA polymerase (RNAP) of Pseudomonas aeruginosa. Utilizing SELEX, we identified and examined aptamers, among which the R2 aptamer demonstrated high specificity and significant binding affinity for RNAP. R2 effectively captured RNAP, making it suitable for protein tandom purification and coating applications. These results have revealed that aptamers are valuable tools for investigating P. aeruginosa RNAP.

UR - http://dx.doi.org/10.1039/d5cc00682a

U2 - 10.1039/d5cc00682a

DO - 10.1039/d5cc00682a

M3 - Article

C2 - 40042155

SN - 1359-7345

VL - 61

SP - 4848

EP - 4851

JO - Chemical Communications

JF - Chemical Communications

IS - 25

ER -

DNA aptamers targeting P. aeruginosa RNAP

Abstract

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