Dna adduct formation of the food carcinogen 2-amino-3-methylimidazo[4, 5-f]quinoline (iq) in liver, kidney and colo-rectum of rats

DNA adducts of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) have been measured in the liver, kidney, and colorectum of male Fischer-344 rats given a single oral dose of IQ (20 mg/kg). The pattern and distribution of DNA adducts examined by ³²P-postlabeling was similar in all tissues. N-(Deoxyguanosin-8-yl)-2-amino-3-methylimidazo-[4,5-f]quinoline (dG-C8-IQ) was the principal adduct identified and it accounted for ∼50-70% of the observed radioactivity, followed by (deoxyguanosin-N²-yl)-2-amino-3-methylimidazo[4,5-f]quinoline (dG-N²-IQ) which accounted for 15-20% of the radioactivity. Twenty-four hours after IQ treatment, DNA modification was greatest in the liver at a level of 7.64 ± 1.08 adducts per 10⁷ bases, followed by kidney at 2.04 ± 0.32 adducts per 10⁷ bases, and colorectum at 1.08 ± 0.22 adducts per 10⁷ bases. Liver and colo-rectum are target tissues of tumorigenesis in the rat during chronic feeding studies with IQ; however, tumors are not formed in the kidney. Therefore, factors in addition to IQ-guanine adduct formation, such as adduct persistence, error-prone repair, and tumor promotion must contribute to organ susceptibility of IQ-induced carcinogenesis.