Chronic rhinosinusitis (CRS) affects nearly all individuals with cystic fibrosis (CF) and is thought to serve as a reservoir for microbiota that subsequently colonize the lung. To better understand the microbial ecology of CRS, we generated a 16S rRNA gene sequencing profile of sinus mucus from CF-CRS patients. We show that CF-CRS sinuses harbor bacterial diversity not entirely captured by clinical culture. Culture data consistently identified the dominant organism in most patients, though lower abundance bacteria were not always identified. We also demonstrate that bacterial communities dominated by Staphylococcus spp. were significantly more diverse compared to those dominated by Pseudomonas spp. Diversity was not significantly associated with clinical factors or patient age, however, younger subjects yielded a much wider range of bacterial diversity. These data mirror bacterial community dynamics in the lung and provide additional insight into the role of sinus microbiota in chronic airway disease progression.
Bibliographical noteFunding Information:
This work was supported by an Investigator-Sponsored Research Grant from Gilead Sciences to RCH and a National Institutes of Health T32 Fellowship (# T90 DE 0227232 ) awarded through the National Institute of Dental and Craniofacial Research to SKL. We thank Abayo Itabiyi and Ali Stockness of the Department of Otolaryngology, members of UMN BioNet for sample acquisition, and the UMN Genomics Center for sequencing assistance.
© 2021 European Cystic Fibrosis Society
- Chronic sinusitis
- Microbial ecology
- Pseudomonas aeruginosa
- Staphylococcus aureus
PubMed: MeSH publication types
- Comparative Study
- Journal Article
- Research Support, N.I.H., Extramural
- Research Support, Non-U.S. Gov't