Diverse patterns of constitutive and inducible overexpression of detoxifying enzyme genes among resistant Aphis glycines populations

Débora Pires Paula, James Menger, David A. Andow, Robert L. Koch

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Understanding the mechanisms of pyrethroid resistance is essential to the effective management of pesticide resistance in Aphis glycines Matsumura (Hemiptera: Aphididae). We mined putative detoxifying enzyme genes in the draft genome sequence of A. glycines for cytochrome oxidase P450 (CYP), glutathione-S-transferase (GST) and esterases (E4 and carboxylesterases-CES). Aphids from clonal populations resistant to pyrethroids from three sites in Minnesota, USA, were screened against a diagnostic LC99 concentration of either λ-cyhalothrin or bifenthrin and detoxifying enzyme genes expression in survivors was analyzed by qPCR. Their expression profiles were compared relative to a susceptible clonal population. We found 61 CYP (40 full-length), seven GST (all full-length), seven E4 (five full-length) and three CES (two full-length) genes, including 24 possible pseudogenes. The detoxifying enzymes had different expression profiles across resistant aphid populations, possibly reflecting differences in the genetic background and pyrethroid selection pressures as the number of constitutively overexpressed detoxifying enzyme genes was correlated with the level of resistance. Our findings will strengthen the understanding of the pyrethroid resistance mechanisms in A. glycines.

Original languageEnglish (US)
Pages (from-to)100-114
Number of pages15
JournalPesticide Biochemistry and Physiology
StatePublished - Mar 2020

Bibliographical note

Funding Information:
We are grateful for the support in the CYP nomenclature provided by Professor David Nelson from the University of Tennessee Health Science Center. This research was partially supported by the Minnesota Soybean Research and Promotion Council and the University of Minnesota Rapid Agricultural Response Fund . Appendix A

Publisher Copyright:
© 2020 Elsevier Inc.


  • Cytochrome P450 monooxygenases
  • Esterases
  • Glutathione S-transferases (GST)
  • Soybean aphid

PubMed: MeSH publication types

  • Journal Article


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