Distribution of integrin subunits in human diabetic kidneys

Dong Kyu Jin, Alfred J. Fish, Elizabeth A. Wayner, Michael Mauer, Suman Setty, Effie-Photini C Tsilibary, Youngki Kim

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Integrins are cell-surface protein receptors that participate in cell adhesion to multiple extracellular matrix ligands, and consist of a and β chain heterodimers. This study examined altered integrin distribution in diabetic nephropathy by investigating 12 human diabetic kidney biopsies, which were compared with normal human kidney. Diabetic nephropathy is characterized by mesangial expansion and progressive thickening of the glomerular basement membrane. Based on morphometric studies of mesangial expansion, diabetic nephropathy was determined to be moderate or severe. Three different patterns (P) of altered intensity of integrin staining were observed. In the mesangial integrin P, the intensity of integrin subunit staining of mesangial cells (α1, α2, α3, β1, αv, αvβ5) was increased in moderate diabetic nephropathy and further increased in severe diabetic nephropathy. In the epithelial integrin P, integrin subunits localized to epithelial cells (αv, β3, αvβ3, αvβ5) were increased to the same extent in moderate and severe diabetic nephropathy. In the endothelial integrin P, integrin subunits localized to endothelial cells (α3, α5, α6, β1) were increased in moderate diabetic nephropathy but returned to normal kidney staining intensity in severe diabetic nephropathy. From these observations, it was concluded that there is significant alteration in the expression of integrin subunits in diabetic nephropathy that is related to the severity of diabetic mesangial expansion. Additionally, the spectrum of integrin subunit alteration appears to be unique to individual glomerular cell types. Given the role of integrins in cell-surface interactions with extracellular matrix components, abnormalities in the expression of these molecules may be important in the pathogenesis of diabetic nephropathy.

Original languageEnglish (US)
Pages (from-to)2636-2645
Number of pages10
JournalJournal of the American Society of Nephrology
Volume7
Issue number12
StatePublished - Dec 1 1996

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Integrins
Diabetic Nephropathies
Kidney
Staining and Labeling
Extracellular Matrix
Glomerular Basement Membrane
Mesangial Cells
Cell Surface Receptors
Cell Adhesion
Cell Communication
Membrane Proteins
Endothelial Cells
Epithelial Cells
Ligands
Biopsy

Keywords

  • Cell adhesion receptors
  • Extracellular matrix
  • Glomerular basement membrane
  • Integrin heterodimers
  • Mesangium

Cite this

Jin, D. K., Fish, A. J., Wayner, E. A., Mauer, M., Setty, S., Tsilibary, E-P. C., & Kim, Y. (1996). Distribution of integrin subunits in human diabetic kidneys. Journal of the American Society of Nephrology, 7(12), 2636-2645.

Distribution of integrin subunits in human diabetic kidneys. / Jin, Dong Kyu; Fish, Alfred J.; Wayner, Elizabeth A.; Mauer, Michael; Setty, Suman; Tsilibary, Effie-Photini C; Kim, Youngki.

In: Journal of the American Society of Nephrology, Vol. 7, No. 12, 01.12.1996, p. 2636-2645.

Research output: Contribution to journalArticle

Jin, DK, Fish, AJ, Wayner, EA, Mauer, M, Setty, S, Tsilibary, E-PC & Kim, Y 1996, 'Distribution of integrin subunits in human diabetic kidneys', Journal of the American Society of Nephrology, vol. 7, no. 12, pp. 2636-2645.
Jin, Dong Kyu ; Fish, Alfred J. ; Wayner, Elizabeth A. ; Mauer, Michael ; Setty, Suman ; Tsilibary, Effie-Photini C ; Kim, Youngki. / Distribution of integrin subunits in human diabetic kidneys. In: Journal of the American Society of Nephrology. 1996 ; Vol. 7, No. 12. pp. 2636-2645.
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