Distribution and dynamics of nitrogen and microbial plankton in southern Lake Michigan during spring transition 1999-2000

Wayne S. Gardner, Peter J. Lavrentyev, Joann F. Cavaletto, Mark J. McCarthy, Brian J. Eadie, Thomas H. Johengen, James B. Cotner

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32 Scopus citations

Abstract

Ammonium and amino acid fluxes were examined as indicators of N and microbial food web dynamics in southern Lake Michigan during spring. Either 15NH+4 or a mixture of 15N-labelled amino acids (both at 4 μM N final concentration) was added to Lake Michigan water. Net fluxes were measured over 24 h under natural light and dark conditions using deck-top incubators and compared to microbial food web characteristics. Isotope dilution experiments showed similar light and dark NH+4 regeneration rates at lake (6 versus 5 nM N h-1) and river-influenced (20 versus 24 nM N h-1) sites. Ammonium uptake rates were similar to regeneration rates in dark bottles. Dark uptake (attributed mainly to bacteria) accounted for ∼70% of total uptake (bacteria plus phytoplankton) in the light at most lake sites but only ∼30% of total uptake at river-influenced sites in or near the St. Joseph River mouth (SJRM). Cluster analysis grouped stations having zero, average, or higher than average N-cycling rates. Discriminant analysis indicated that chlorophyll concentration, oligotrich ciliate biomass, and total P concentration could explain 66% of N-cycling rate variation on average. Heterotrophic bacterial N demand was about one third of the NH+4 regeneration rate. Results suggest that, with the exception of SJRM stations, bacterial uptake and protist grazing mediated much of the N dynamics during spring transition. Since NH+4 is more available to bacteria than NO-3, regenerated NH+4 may have a strong influence on spring, lake biochemical energetics by enhancing N-poor organic matter degradation in this NO-3 -replete ecosystem.

Original languageEnglish (US)
Pages (from-to)C03007 1-16
JournalJournal of Geophysical Research: Oceans
Volume109
Issue number3
DOIs
StatePublished - Mar 15 2004

Keywords

  • Lake Michigan
  • Microbial food web
  • Nitrogen

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