Distribution and developmentally regulated expression of murine polycystin

Yoav Segal, Anna Pavlova, Elvino J G Barros, Corinna Löhning, L. U. Weining, Sanjay K. Nigam, Anna Maria Frischauf, Stephen T. Reeders, Jing Zhou

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

-PKDl, the gene that is mutated in -85% of autosomal dominant polycystic kidney disease (ADPKD) cases in humans, has recently been identified (Eur. PKD Consortium. Cell 77: 881-894, 1994; also, erratum in Cell 78: 1994). The longest open-reading frame of PKD1 encodes polycystin, a novel ∼460-kDa protein that contains a series of NH2-terminal adhesive domains (J. Hughes, C. J. Ward, B. Peral, R. Aspinwall, K. Clark, J. San Millan, V. Gamble, and P. C. Harris. Nat. Genet. 10: 151-160, 1995; and Int. PKD Consortium. Cell 81: 289-298, 1995) and several putative transmembrane segments. To extend studies of polycystin to an experimentally accessible animal, we have isolated a cDNA clone encoding the 3′ end of Pkdl, the mouse homologue of PKD1, and raised a specific antibody to recombinant murine polycystin. This antibody was used to determine the subcellular localization and tissue distribution of the protein by Western analysis and immunocytochemistry. In the mouse, polycystin is an ∼400-kDa molecule that is predominantly found in membrane fractions of tissue and cell extracts. It is expressed in many tissues including kidney, liver, pancreas, heart, intestine, lung, and brain. Renal expression, which is confined to tubular epithelia, is highest in late fetal and early neonatal life and drops 20-fold by the third postnatal week, maintaining this level into adulthood. Thus the temporal profile of polycystin expression coincides with kidney tubule differentiation and maturation.

Original languageEnglish (US)
Pages (from-to)F451-P459
JournalAmerican Journal of Physiology
Volume272
Issue number4 PART 2
DOIs
StatePublished - 1997

Keywords

  • Immunohistochemistry
  • Kidney development
  • PKD1
  • Polycystic kidney disease
  • Western analysis

Fingerprint Dive into the research topics of 'Distribution and developmentally regulated expression of murine polycystin'. Together they form a unique fingerprint.

Cite this