We developed a 16S rRNA-targeted oligonucleotide probe (S-P-GPos-1200-a-A-13) for the Gram-positive bacteria, confirmed its specificity by database searches and hybridization studies, and investigated the effects of humic acids on membrane hybridizations with this probe. S-P-GPos-1200-a-A-13 was used to estimate the abundance of Gram-positive populations in the bovine rumen and Lake Michigan sediments. This probe should be useful for studies of the environmental distribution of Gram-positive bacteria and the detection of uncultured, phylogenetically Gram-positive bacteria with variable or negative Gram staining reactions, and could serve for Gram staining in some diagnostic settings.
Bibliographical noteFunding Information:
This work was supported by NSF Grant DEB-9615356. The Lake Michigan sediment samples were collected during cruises on the R/V Neeskay, for which we thank Captain Ron Smith, First Mate Greg Stamatelakys, Duane Moser, and the crew. We also thank the Center for Great Lakes Studies of the University of Wisconsin-Milwaukee for laboratory facilities.
Copyright 2007 Elsevier B.V., All rights reserved.
- 16S rRNA
- Gram variable bacteria
- Gram-positive bacteria
- Humic acid
- Lake Michigan
- Oligonucleotide hybridization probe