Distinguishing tumor cells via analyzing intracellular telomerase activity

Lin Chen, Junyi Huang, Fanben Meng, Nandi Zhou

Research output: Contribution to journalArticlepeer-review

Abstract

Two strategies to identify tumor cells were developed in this research via analyzing the activity of telomerase inside the cells, which is regarded as the mark enzyme of tumors. The lysate of cells was used to assay directly. In the electrochemical assay, the substrate of telomerase was incubated with the lysate, followed by PCR amplification of the elongated products. Then the oxidation peak current of guanine that existed in the hydrolyte of PCR products was used as a target to identify tumor cells. In the spectral assay, the lysate of cells was used to catalyze the elongation of the substrate of telomerase modified on the surface of gold nanoparticles. Due to the formation of G-quadruple structures after elongation and subsequent aggregation of gold nanoparticles, the changes in UV-visible spectra can also be utilized to identify tumor cells. 2010

Original languageEnglish (US)
Pages (from-to)535-538
Number of pages4
JournalAnalytical Sciences
Volume26
Issue number5
DOIs
StatePublished - May 2010

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