Distinct volume distribution of viable and non-viable hybridoma cells: A flow cytometric study

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18 Scopus citations

Abstract

Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. The distribution based on small-angle light scattering is similar to the distribution based on volume as measured by Elzone particle counter. It thus appears that viable cells form the population with a larger mean cell volume. The results also indicate that the volume of viable cells decreases during the cultivation while that of dead cells remains relatively constant.

Original languageEnglish (US)
Pages (from-to)85-94
Number of pages10
JournalCytotechnology
Volume2
Issue number2
DOIs
StatePublished - May 1 1989

Keywords

  • cell culture
  • cell volume
  • flow cytometry
  • hybridoma

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