Distinct carbon fractions drive a generalisable two-pool model of fungal necromass decomposition

Craig R. See, Chris W. Fernandez, Anna M. Conley, Lang C. DeLancey, Katherine A. Heckman, Peter G. Kennedy, Sarah E. Hobbie

Research output: Contribution to journalArticlepeer-review

Abstract

Fungi represent a rapidly cycling pool of carbon (C) and nitrogen (N) in soils. Understanding of how this pool impacts soil nutrient availability and organic matter fluxes is hindered by uncertainty regarding the dynamics and drivers of fungal necromass decomposition. Here we assessed the generality of common models for predicting mass loss during fungal necromass decomposition and linked the resulting parameters to necromass substrate chemistry. We decomposed 28 different types of fungal necromass in laboratory microcosms over a 90-day period, measuring mass loss on all types, and N release on a subset of types. We characterised the initial chemistry of each necromass type using: (a) fibre analysis methods commonly used for plant tissues, (b) initial melanin and nitrogen (N) concentrations and (c) Fourier transform infrared (FTIR) spectroscopy to assess the presence of bonds associated with common biomolecules. We found universal support for an asymptotic model of decomposition, which assumes that fungal necromass consists of an exponentially decomposing ‘fast’ pool, and a ‘slow’ pool that decomposes at a rate approaching zero. The strongest predictor of the fast pool decay rate (k) was the proportion of cell soluble components, though initial N concentration also predicted k, albeit more weakly. The size of the slow pool was best predicted by the acid non-hydrolysable fraction, which was positively correlated with melanin-associated aromatics. Nitrogen dynamics varied by necromass type, ranging from net N release to net immobilisation. The maximum quantity of N immobilised was inversely related to cell soluble contents and k, as positively related to FTIR spectra associated with cell wall polysaccharides. Collectively, our results indicate that the decomposition of fungal necromass in soils can be described as having two distinct stages that are driven by different components of substrate C chemistry, with implications for rates of N availability and organic matter accumulation in soils. A free Plain Language Summary can be found within the Supporting Information of this article.

Original languageEnglish (US)
Pages (from-to)796-806
Number of pages11
JournalFunctional Ecology
Volume35
Issue number3
DOIs
StatePublished - Mar 2021

Bibliographical note

Funding Information:
We thank two anonymous reviewers for their excellent feedback on an earlier version of this manuscript. Hannah Vanderscheuren and Hanan Farah provided additional assistance in the laboratory. This work was funded in part by NSF DEB‐1754679 to P. Kennedy, a Carleton College Externship to A. Conley and a Minnesota Mycological Society graduate research grant to C. See. The paper was written in part during C. See's residency at the Sitka Center for Arts and Ecology. This research is part of the Cedar Creek LTER Program, funded by NSF DEB‐1234162 and DEB‐1831944.

Publisher Copyright:
© 2020 British Ecological Society

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

Keywords

  • carbon cycling
  • hyphal turnover
  • microbial biomass
  • nitrogen cycling
  • nitrogen immobilisation
  • soil carbon
  • soil organic matter

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