Dissection of the stepwise mechanism to β-lactam formation and elucidation of a rate-determining conformational change in β-lactam synthetase

Keyphrases

• Lactams 100%
• Conformational Change 100%
• Synthetase 100%
• Kcat 100%
• Rate-determining Step 100%
• Stepwise Mechanism 100%
• Lactam Formation 100%
• Solvent Isotope Effect 60%
• Adenylation 40%
• Acyl 40%
• Site-directed mutation 20%
• Active Sites 20%
• Catalytic Cycle 20%
• Heavy Water 20%
• Substrate Binding 20%
• Rate Limiting 20%
• Product Release 20%
• Active Loop 20%
• Viscosity Test 20%
• Second-order Rate Constant 20%
• Protein Conformational Change 20%
• Kramers Theory 20%
• β-lactamase Inhibitor 20%
• Ionizable Groups 20%
• Torsional Strain 20%
• Internal Friction 20%
• Clavulanic Acid 20%
• Loop Movement 20%
• Chemical Barrier 20%
• Lactose Synthesis 20%
• Eyring Plot 20%

Biochemistry, Genetics and Molecular Biology

• Conformational Change 100%
• Turnover Number 100%
• Active Site 40%
• Adenylylation 40%
• Mechanical Torsion 20%
• Enzyme 20%
• Adenosine Triphosphate 20%

Chemistry

• Lactam 100%
• formation 100%
• Kcat 50%
• Isotope Effect 30%
• ATP 10%
• Rate Constant 10%
• Clavulanic Acid 10%
• catalytic cycle 10%