Since more than a decade ago, Saccharomyces cerevisiae has been used as a model to dissect complex traits, revealing the genetic basis of a large number of traits in fine detail. However, to have a more global view of the genetic architecture of traits across species, the examination of the molecular basis of phenotypes within non-conventional species would undoubtedly be valuable. In this respect, the Saccharomycotina yeasts represent ideal and potential non-model organisms. Here we sought to assess the feasibility of genetic mapping by bulk segregant analysis in the protoploid Lachancea kluyveri (formerly S. kluyveri) yeast species, a distantly related species to S. cerevisiae. For this purpose, we designed a fluorescent mating-type marker, compatible with any mating-competent strains representative of this species, to rapidly create a large population of haploid segregants (>105 cells). Quantitative trait loci can be mapped by selecting and sequencing an enriched pool of progeny with extreme phenotypic values. As a test bed, we applied this strategy and mapped the causal loci underlying halotolerance phenotypes in L. kluyveri. Overall, this study demonstrates that bulk segregant mapping is a powerful way for investigating the genetic basis of natural variations in non-model yeast organisms and more precisely in L. kluyveri.
Bibliographical noteFunding Information:
Thisworkwas supported by an ANR grant (2010-BLAN-1606) (GF, BL and JS) and an ANR Young Investigator grant (2011-JSV6-004-01) (JS). We also thank the Universit? de Strasbourg (IdEx 2012 Attractivit?) for their financial support and the Bioimage platform for their support. JH is supported in part by a grant from the Minist?re de l'Enseignement Sup?rieur et de la Recherche and in part by a fellowship from the medical association La Ligue contre le Cancer. AS is supported by a grant from R?gion Alsace.
- Lachancea kluyveri
- Quantitative traits
- Trait mapping