Disease course in mdx:Utrophin+/− mice

comparison of three mouse models of duchenne muscular dystrophy

Abby A. McDonald, Sadie L Hebert, Matthew D. Kunz, Steven J. Ralles, Linda K McLoon

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The mdx mouse model of Duchenne muscular dystrophy (DMD) is used to study disease mechanisms and potential treatments, but its pathology is less severe than DMD patients. Other mouse models were developed to more closely mimic the human disease based on knowledge that upregulation of utrophin has a protective effect in mdx muscle. An mdx:utrophin−/− (dko) mouse was created, which had a severe disease phenotype and a shortened life span. An mdx:utrophin+/‒ mouse was also created, which had an intermediate disease phenotype compared to the mdx and dko mice. To determine the usefulness of mdx:utrophin+/‒ mice for long-term DMD studies, limb muscle pathology and function were assessed across the life span of wild-type, mdx, mdx:utrophin+/‒, and dko mice. Muscle function assessment, specifically grip duration and rotarod performance, demonstrated that mdx:utrophin+/‒ mice were weaker for a longer time than mdx mice. Mean myofiber area was smaller in mdx:utrophin+/‒ mice compared to mdx mice at 12 months. Mdx: utrophin+/‒ mice had a higher percentage of centrally nucleated myofibers compared to mdx mice at 6 and 12 months. Collagen I and IV density was significantly higher in mdx:utrophin+/‒ muscle compared to mdx at most ages examined. Generally, mdx:utrophin+/‒ mice showed an intermediate disease phenotype over a longer time course compared to the mdx and dko mice. While they do not genetically mirror human DMD, mdx:utrophin+/‒ mice may be a more useful animal model than mdx or dko mice for investigating long-term efficacy of potential treatments when fibrosis or muscle function is the focus.

Original languageEnglish (US)
Article numbere12391
JournalPhysiological Reports
Volume3
Issue number4
DOIs
StatePublished - Jan 1 2015

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Utrophin
Inbred mdx Mouse
Duchenne Muscular Dystrophy
Muscles
Phenotype
Pathology
Hand Strength

Keywords

  • Collagen
  • Dystrophin
  • Fibrosis
  • Mouse models
  • Muscle disease
  • Muscle function
  • Muscular dystrophy
  • Skeletal muscle
  • Utrophin

Cite this

Disease course in mdx:Utrophin+/− mice : comparison of three mouse models of duchenne muscular dystrophy. / McDonald, Abby A.; Hebert, Sadie L; Kunz, Matthew D.; Ralles, Steven J.; McLoon, Linda K.

In: Physiological Reports, Vol. 3, No. 4, e12391, 01.01.2015.

Research output: Contribution to journalArticle

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abstract = "The mdx mouse model of Duchenne muscular dystrophy (DMD) is used to study disease mechanisms and potential treatments, but its pathology is less severe than DMD patients. Other mouse models were developed to more closely mimic the human disease based on knowledge that upregulation of utrophin has a protective effect in mdx muscle. An mdx:utrophin−/− (dko) mouse was created, which had a severe disease phenotype and a shortened life span. An mdx:utrophin+/‒ mouse was also created, which had an intermediate disease phenotype compared to the mdx and dko mice. To determine the usefulness of mdx:utrophin+/‒ mice for long-term DMD studies, limb muscle pathology and function were assessed across the life span of wild-type, mdx, mdx:utrophin+/‒, and dko mice. Muscle function assessment, specifically grip duration and rotarod performance, demonstrated that mdx:utrophin+/‒ mice were weaker for a longer time than mdx mice. Mean myofiber area was smaller in mdx:utrophin+/‒ mice compared to mdx mice at 12 months. Mdx: utrophin+/‒ mice had a higher percentage of centrally nucleated myofibers compared to mdx mice at 6 and 12 months. Collagen I and IV density was significantly higher in mdx:utrophin+/‒ muscle compared to mdx at most ages examined. Generally, mdx:utrophin+/‒ mice showed an intermediate disease phenotype over a longer time course compared to the mdx and dko mice. While they do not genetically mirror human DMD, mdx:utrophin+/‒ mice may be a more useful animal model than mdx or dko mice for investigating long-term efficacy of potential treatments when fibrosis or muscle function is the focus.",
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