TY - JOUR
T1 - Discovery of UPSTREAM OF FLOWERING LOCUS C (UFC) and FLOWERING LOCUS C EXPRESSOR (FLX) in Gladiolus ×hybridus, G. dalenii
AU - Aljaser, Jaser A.
AU - Anderson, Neil O.
AU - Noyszewski, Andrzej
N1 - Publisher Copyright:
© 2022 by the author(s). Published by Maximum Academic Press.
PY - 2022
Y1 - 2022
N2 - The lack of identified flowering genes in ornamental geophytic crops, such as Gladiolus, is critical to further genetic research. The UPSTREAM OF FLOWERING LOCUS C (UFC) gene is adjacent to FLOWERING LOCUS C (FLC) which is a floral repressor; FLC EXPRESSOR (FLX) upregulates FRIGIDA which upregulates FLC expression. The purpose of this research was to determine whether two flowering genes exist in Gladiolus ×hybridus and G. dalenii: UFC and FLX. Seventeen early flowering and commercial cultivars possess the UFC gene with four exons in two allelic forms. The sequenced UFC gene, when translated into its amino acid sequence and set in pair-alignment to other species, has < 57% in amino acid identity to Musa acuminata. The FLX gene in gladiolus has 3/5 (60%) exons in common with Ananas comosus; pair-alignment of the exons has ~65% identity of FLX to A. comosus. The UFC protein consists of a conserved domain, DUF966, which is higher in identity (86%) and pair-alignment with Elaeis guineensis. The two newly-discovered genes in gladiolus, UFC and FLX, provide insight into the flowering mechanism, flowering pathway genes, and vernalization response.
AB - The lack of identified flowering genes in ornamental geophytic crops, such as Gladiolus, is critical to further genetic research. The UPSTREAM OF FLOWERING LOCUS C (UFC) gene is adjacent to FLOWERING LOCUS C (FLC) which is a floral repressor; FLC EXPRESSOR (FLX) upregulates FRIGIDA which upregulates FLC expression. The purpose of this research was to determine whether two flowering genes exist in Gladiolus ×hybridus and G. dalenii: UFC and FLX. Seventeen early flowering and commercial cultivars possess the UFC gene with four exons in two allelic forms. The sequenced UFC gene, when translated into its amino acid sequence and set in pair-alignment to other species, has < 57% in amino acid identity to Musa acuminata. The FLX gene in gladiolus has 3/5 (60%) exons in common with Ananas comosus; pair-alignment of the exons has ~65% identity of FLX to A. comosus. The UFC protein consists of a conserved domain, DUF966, which is higher in identity (86%) and pair-alignment with Elaeis guineensis. The two newly-discovered genes in gladiolus, UFC and FLX, provide insight into the flowering mechanism, flowering pathway genes, and vernalization response.
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U2 - 10.48130/OPR-2022-0013
DO - 10.48130/OPR-2022-0013
M3 - Article
AN - SCOPUS:85173715594
SN - 2769-2094
VL - 2
JO - Ornamental Plant Research
JF - Ornamental Plant Research
M1 - 13
ER -