Discerning the role of topography and ion exchange in cell response of bioactive tissue engineering scaffolds

Elisabeth Engel, Sergio Del Valle, Conrado Aparicio, George Altankov, Luis Asin, Josep A. Planell, Maria Pau Ginebra

Research output: Contribution to journalArticlepeer-review

63 Scopus citations


Surface topography is known to have an influence on osteoblast activity. However, in the case of bioactive materials, topographical changes can affect also ion exchange properties. This makes the problem more complex, since it is often difficult to separate the strictly topographical effects from the effects of ionic fluctuations in the medium. The scope of this paper is to analyze the simultaneous effect of topography and topography-mediated ion exchange on the initial cellular behavior of osteoblastic-like cells cultured on bioactive tissue engineering substrates. Two apatitic substrates with identical chemical composition but different micro/nanostructural features were obtained by low-temperature setting of a calcium phosphate cement. MG63 osteoblastic-like cells were cultured either in direct contact with the substrates or with their extracts. A strong and permanent decrease of calcium concentration in the culture medium, dependent on substrate topography, was detected. A major effect of the substrate microstructure on cell proliferation was observed, explained in part by the topography-mediated ion exchange, but not specifically by the ionic Ca2+ fluctuations. Cell differentiation was strongly enhanced when cells were cultured on the finer substrate. This effect was not explained by the chemical modification of the medium, but rather suggested a strictly topographical effect.

Original languageEnglish (US)
Pages (from-to)1341-1351
Number of pages11
JournalTissue Engineering - Part A.
Issue number8
StatePublished - Aug 1 2008


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