Directed differentiation and neurite extension of mouse embryonic stem cell on aligned poly(lactide) nanofibers functionalized with YIGSR peptide

Laura A. Smith Callahan, Sibai Xie, Ian A. Barker, Jukuan Zheng, Darrell H. Reneker, Andrew P. Dove, Matthew L. Becker

Research output: Contribution to journalArticlepeer-review

104 Scopus citations

Abstract

End-functional PLLA nanofibers were fabricated into mats of random or aligned fibers and functionalized post-spinning using metal-free "click chemistry" with the peptide Tyr-Ile-Gly-Ser-Arg (YIGSR). Fibers that were both aligned and functionalized with YIGSR were found to significantly increase the fraction of mouse embryonic stem cells (mESC) expressing neuron-specific class III beta-tubulin (TUJ1), the level of neurite extension and gene expression for neural markers compared to mESC cultured on random fiber mats and unfunctionalized matrices. Precise functionalization of degradable polymers with bioactive peptides created translationally-relevant materials that capitalize on the advantages of both synthetic and natural systems, while mitigating the classic limitations of each.

Original languageEnglish (US)
Pages (from-to)9089-9095
Number of pages7
JournalBiomaterials
Volume34
Issue number36
DOIs
StatePublished - Dec 2013

Bibliographical note

Funding Information:
The authors are grateful for financial support from the Akron Functional Materials Center and the EPSRC for a postdoctoral fellowship for I.A.B. ( EP/I014454/01 ). The SECs used in this research were obtained through Birmingham Science City: Innovative Uses for Advanced Materials in the Modern World (West Midlands Centre for Advanced Materials Project 2), with support from Advantage West Midlands (AWM) and partly funded by the European Regional Development Fund (ERDF). We also thank Purac for the donation of l -lactide.

Keywords

  • Electrospinning
  • Nanofiber
  • Neural differentiation
  • Polymer
  • YIGSR

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