Directed cell migration towards softer environments

Aleksi Isomursu, Keun-young Park, Jay Hou, Bo Cheng, Mathilde Mathieu, Ghaidan A Shamsan, Ben Fuller, Jesse Kasim, M. Mohsen Mahmoodi, Tian Jian Lu, Guy M. Genin, Feng Xu, Min Lin, Mark D. Distefano, Johanna Ivaska, David J. Odde

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

How cells sense tissue stiffness to guide cell migration is a fundamental question in development, fibrosis and cancer. Although durotaxis—cell migration towards increasing substrate stiffness—is well established, it remains unknown whether individual cells can migrate towards softer environments. Here, using microfabricated stiffness gradients, we describe the directed migration of U-251MG glioma cells towards less stiff regions. This ‘negative durotaxis’ does not coincide with changes in canonical mechanosensitive signalling or actomyosin contractility. Instead, as predicted by the motor–clutch-based model, migration occurs towards areas of ‘optimal stiffness’, where cells can generate maximal traction. In agreement with this model, negative durotaxis is selectively disrupted and even reversed by the partial inhibition of actomyosin contractility. Conversely, positive durotaxis can be switched to negative by lowering the optimal stiffness by the downregulation of talin—a key clutch component. Our results identify the molecular mechanism driving context-dependent positive or negative durotaxis, determined by a cell’s contractile and adhesive machinery.

Original languageEnglish (US)
JournalNature Materials
DOIs
StateAccepted/In press - 2022

Bibliographical note

Funding Information:
We thank L. S. Prahl, J. Tian and G. Huang for helpful discussions on computational modelling and members of the Ivaska Lab for their insightful comments and discussion. Simulations were run in part on the high-performance computing resources at the Minnesota Supercomputing Institute. Turku Bioscience Centre Cell Imaging and Cytometry Core and Biocenter Finland are acknowledged for their services, instrumentation and expertise. We are supported by the University of Turku Doctoral Programme in Molecular Life Sciences (A.I.), the Company of Biologists Travelling Fellowship (A.I.), the Finnish Cultural Foundation (A.I.), the Academy of Finland (AoF CoE 346131 and 325464 (J.I.)), ERC CoG (grant 615258 (J.I.)), Sigrid Juselius Foundation (J.I.), the Finnish Cancer Organization (J.I.), the National Natural Science Foundation of China (11972280 (F.X.); 11772253 (M.L.); 12022206 (M.L.); 11532009 (T.J.L.); 12002262 (B.C.)), Natural Science Basic Research Plan in Shaanxi Province of China (2022KWZ-17 (M.L.)), the Shaanxi Province Youth Talent Support Program (M.L.), the Young Talent Support Plan of Xi’an Jiaotong University (M.L.), the National Institutes of Health (R01 AR077793 (G.M.G.); R01 CA172986 (D.J.O.); U54 CA210190 (D.J.O.); P01 CA254849 (D.J.O.); R35GM141853 (M.D.D.)) and the NSF Science and Technology Center for Engineering Mechanobiology (CMMI 1548571 (G.M.G.)).

Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature Limited.

PubMed: MeSH publication types

  • Journal Article

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