The direct synthesis method is a relatively simple approach for generating modified oligonucleotides of defined base sequence with site-specifically placed polycyclic aromatic hydrocarbon diol epoxide-modified deoxyguanosyl and deoxyadenosyl residues. Up to 5-10 mg quantities of several different modified oligonucleotides have been synthesized for detailed NMR structural studies. Smaller amounts of modified oligonucleotides containing a single modified guanosyl residue in oligonucleotides 10-11 bases long containing up to three other unmodified guanine residues can be readily generated in smaller quantities for site-directed mutagenesis and other studies.
|Original language||English (US)|
|Number of pages||8|
|Journal||Polycyclic Aromatic Compounds|
|State||Published - 1996|
Bibliographical noteFunding Information:
This work was supported by the Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Grant CA 57226.
- NMR structures
- PAH diol epoxides
- Site-specific PAH-DNA adducts