Direct subcutaneous injection of polyethylene particles over the murine calvaria results in dramatic osteolysis

Allison J. Rao, Stefan Zwingenberger, Roberto Valladares, Chenguang Li, Robert Lane Smith, Stuart B. Goodman, Christophe Nich

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Purpose: The murine calvarial model has been widely employed for the in vivo study of particle-induced osteolysis, the most frequent cause of aseptic loosening of total joint replacements. Classically, this model uses an open surgical technique in which polyethylene (PE) particles are directly spread over the calvarium for the induction of osteolysis. We evaluated a minimally invasive modification of the calvarial model by using a direct subcutaneous injection of PE particles. Methods: Polyethylene (PE) particles were injected subcutaneously over the calvaria of C57BL6J ten-week-old mice ("injection" group) or were implanted after surgical exposure of the calvaria ("open" group) (n = 5/group). For each group, five additional mice received no particles and served as controls. Particle-induced osteolysis was evaluated two weeks after the procedure using high-definition microCT imaging. Results: Polyethylene particle injection over the calvaria resulted in a 40 % ± 1.8 % decrease in the bone volume fraction (BVF), compared to controls. Using the "open surgical technique", the BVF decreased by 16 % ± 3.8 % as compared to controls (p < 0.0001). Conclusions: Direct subcutaneous injection of PE particles over the murine calvaria produced more profound resorption of bone. Polyethylene particle implantation by injection is less invasive and reliably induces osteolysis to a greater degree than the open technique. This subcutaneous injection method will prove useful for repetitive injections of particles, and the assessment of potential local or systemic therapies.

Original languageEnglish (US)
Pages (from-to)1393-1398
Number of pages6
JournalInternational Orthopaedics
Issue number7
StatePublished - Jul 2013
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgements We would like to thank the Ellenburg Chair of Surgery and the Medical Scholars Program at the Stanford University School of Medicine, la Société Française de Chirurgie Orthopédique (SOFCOT), L’Assistance Publique – Hôpitaux de Paris (AP-HP), and Le Centre National de la Recherche Scientifique (CNRS).


Dive into the research topics of 'Direct subcutaneous injection of polyethylene particles over the murine calvaria results in dramatic osteolysis'. Together they form a unique fingerprint.

Cite this