Direct quantitation of poly(ADP-Ribose) polymerase (PARP) activity as a means to distinguish necrotic and apoptotic death in cell and tissue samples

Karson S. Putt; Gregory J. Beilman; Paul J. Hergenrother

doi:10.1002/cbic.200400330

Direct quantitation of poly(ADP-Ribose) polymerase (PARP) activity as a means to distinguish necrotic and apoptotic death in cell and tissue samples

Karson S. Putt
, Gregory J. Beilman
, Paul J. Hergenrother

Administration (TMED)

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

PARP-angel of death. The PARP-1 enzyme is either inactivated or activated depending on whether a cell dies via apoptosis or necrosis (see scheme). This differential processing of PARP-1 can now be used as a rapid, sensitive, and convenient method to distinguish apoptotic from necrotic cell death, and is even applied to quantitate necrosis in bulk tissue from a pig in which a hemorrhagic shock was induced.

Original language	English (US)
Pages (from-to)	53-55
Number of pages	3
Journal	ChemBioChem
Volume	6
Issue number	1
DOIs	https://doi.org/10.1002/cbic.200400330
State	Published - Jan 2005

Keywords

Apoptosis
Bioorganic chemistry
Enzymes
Necrosis
PARP-1

Publisher link

10.1002/cbic.200400330

Find It

Find It at U of M Libraries

Cite this

@article{b8936b2d17764b7499fc2f06fa9c2914,

title = "Direct quantitation of poly(ADP-Ribose) polymerase (PARP) activity as a means to distinguish necrotic and apoptotic death in cell and tissue samples",

abstract = "PARP-angel of death. The PARP-1 enzyme is either inactivated or activated depending on whether a cell dies via apoptosis or necrosis (see scheme). This differential processing of PARP-1 can now be used as a rapid, sensitive, and convenient method to distinguish apoptotic from necrotic cell death, and is even applied to quantitate necrosis in bulk tissue from a pig in which a hemorrhagic shock was induced.",

keywords = "Apoptosis, Bioorganic chemistry, Enzymes, Necrosis, PARP-1",

author = "Putt, \{Karson S.\} and Beilman, \{Gregory J.\} and Hergenrother, \{Paul J.\}",

year = "2005",

month = jan,

doi = "10.1002/cbic.200400330",

language = "English (US)",

volume = "6",

pages = "53--55",

journal = "ChemBioChem",

issn = "1439-4227",

publisher = "John Wiley and Sons Inc.",

number = "1",

}

TY - JOUR

T1 - Direct quantitation of poly(ADP-Ribose) polymerase (PARP) activity as a means to distinguish necrotic and apoptotic death in cell and tissue samples

AU - Putt, Karson S.

AU - Beilman, Gregory J.

AU - Hergenrother, Paul J.

PY - 2005/1

Y1 - 2005/1

N2 - PARP-angel of death. The PARP-1 enzyme is either inactivated or activated depending on whether a cell dies via apoptosis or necrosis (see scheme). This differential processing of PARP-1 can now be used as a rapid, sensitive, and convenient method to distinguish apoptotic from necrotic cell death, and is even applied to quantitate necrosis in bulk tissue from a pig in which a hemorrhagic shock was induced.

AB - PARP-angel of death. The PARP-1 enzyme is either inactivated or activated depending on whether a cell dies via apoptosis or necrosis (see scheme). This differential processing of PARP-1 can now be used as a rapid, sensitive, and convenient method to distinguish apoptotic from necrotic cell death, and is even applied to quantitate necrosis in bulk tissue from a pig in which a hemorrhagic shock was induced.

KW - Apoptosis

KW - Bioorganic chemistry

KW - Enzymes

KW - Necrosis

KW - PARP-1

UR - https://www.scopus.com/pages/publications/12344324970

UR - https://www.scopus.com/pages/publications/12344324970#tab=citedBy

U2 - 10.1002/cbic.200400330

DO - 10.1002/cbic.200400330

M3 - Article

C2 - 15549722

AN - SCOPUS:12344324970

SN - 1439-4227

VL - 6

SP - 53

EP - 55

JO - ChemBioChem

JF - ChemBioChem

IS - 1

ER -

Direct quantitation of poly(ADP-Ribose) polymerase (PARP) activity as a means to distinguish necrotic and apoptotic death in cell and tissue samples

Abstract

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this