Direct quantitation of poly(ADP-Ribose) polymerase (PARP) activity as a means to distinguish necrotic and apoptotic death in cell and tissue samples

Karson S. Putt, Gregory J. Beilman, Paul J. Hergenrother

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

PARP-angel of death. The PARP-1 enzyme is either inactivated or activated depending on whether a cell dies via apoptosis or necrosis (see scheme). This differential processing of PARP-1 can now be used as a rapid, sensitive, and convenient method to distinguish apoptotic from necrotic cell death, and is even applied to quantitate necrosis in bulk tissue from a pig in which a hemorrhagic shock was induced.

Original languageEnglish (US)
Pages (from-to)53-55
Number of pages3
JournalChemBioChem
Volume6
Issue number1
DOIs
StatePublished - Jan 1 2005

Keywords

  • Apoptosis
  • Bioorganic chemistry
  • Enzymes
  • Necrosis
  • PARP-1

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