Direct immobilization of manganese chelates on silica nanospheres for MRI applications

Marcell Pálmai, Adrienn Pethő, Lívia Naszályi Nagy, Szilvia Klébert, Zoltán May, Judith Mihály, András Wacha, Katalin Jemnitz, Zsuzsanna Veres, Ildikó Horváth, Krisztián Szigeti, Domokos Máthé, Zoltán Varga

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The development of tissue specific magnetic resonance imaging (MRI) contrast agents (CAs) is very desirable to achieve high contrast ratio combined with excellent anatomical details. To this end, we introduce a highly effective manganese(II) containing silica material, with the aim to shorten the longitudinal (T 1 ) relaxation time. The microporous silica nanospheres (MSNSs) with enlarged porosity and specific surface area were prepared by a surfactant assisted aqueous method. Subsequently, the surface silanol groups were amino-functionalized, reacted with diethylenetriaminepentaacetic (DTPA) dianhydride and finally deposited with Mn 2+ . After comprehensive characterization, the MRI properties of functionalized MSNSs were investigated. The resulting nanospheres demonstrated substantial contrast enhancement during the in vitro MRI investigations, which was also evidenced by significant contrast enhancement on T 1 -weighted MR images in vivo. Moreover, in vitro cytotoxicity assay of functionalized MSNSs on hepatocyte mono- and hepatocyte-Kuppfer cell co-cultures showed no significant decrease in cell viability. Our findings confirmed our hypothesis, that Mn 2+ -chelating MSNSs are appropriate candidates for liver-specific T 1 -weighted MRI CAs with high relaxivities (r 1  = 7.18 mM −1  s −1 ).

Original languageEnglish (US)
Pages (from-to)298-305
Number of pages8
JournalJournal of Colloid And Interface Science
Volume498
DOIs
StatePublished - Jul 15 2017

Bibliographical note

Funding Information:
The authors gratefully acknowledge Ter?z Kiss for TEM images. The author (KSZ) was supported by the J?nos Bolyai Research Fellowship program of the Hungarian Academy of Sciences.

Publisher Copyright:
© 2017 Elsevier Inc.

Keywords

  • DTPA
  • Manganese(II) chelate
  • Nanoparticulate MRI contrast agent
  • Silica nanospheres

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