Direct evidence for intrastrand DNA inversion of X immunoglobulin gene segments in two murine plasmacytomas

Rodney Feddersen, Brian van Ness

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The products of kappa immunoglobulin gene recombination have been characterized in two murine plasmacytomas to examine the relationship between V-J products and reciprocal elements. By cloning, sequencing, hybridization, and application of the polymerase chain reaction, we have established the direct relationship of the kappa recombination products in these cells. The results provide stronger support for the intrastrand mechanism of kappa gene recombination as well as demonstrating a role for secondary, corrective recombinations.

Original languageEnglish (US)
Pages (from-to)9797-9809
Number of pages13
JournalNucleic acids research
Volume17
Issue number23
DOIs
StatePublished - Dec 11 1989

Bibliographical note

Funding Information:
We thank M. Weigert for providing plasmacytoma DNA. This work was supported by Grant GM 37687 from the National Institutes of Health.

Fingerprint Dive into the research topics of 'Direct evidence for intrastrand DNA inversion of X immunoglobulin gene segments in two murine plasmacytomas'. Together they form a unique fingerprint.

Cite this