Direct determination of total serum phospholipid by X ray fluorescence

D. E. Leyden, P. W. Carr, W. K. Nonidez

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An X ray fluorescence method for measurement of total serum phospholipid is described. A Folch extract of 1 ml of serum was evaporated and deposited on a paper disc by washing with chloroform. A coefficient of variation of 4% was obtained for samples containing at least 1 micromole of lipid, as phosphorus. The major advantage of the method is its speed (5 min/sample) in comparison to the phosphomolybdenum blue procedure. The method is free of interference from other lipid constituents and the phosphorous content can be standardized by comparison with very stable inorganic phosphates.

Original languageEnglish (US)
Pages (from-to)1271-1274
Number of pages4
JournalClinical chemistry
Issue number10
StatePublished - 1974


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