DIRECT DETECTION OF HIV RNA EXPRESSION IN SEROPOSITIVE SUBJECTS

Clyde Hart; Thomas Spira; Jennifer Moore; John Sninsky; Gerald Schochetman; Alan R Lifson; Judith Galphin; Chin Yih Ou

doi:10.1016/S0140-6736(88)90639-3

DIRECT DETECTION OF HIV RNA EXPRESSION IN SEROPOSITIVE SUBJECTS

Clyde Hart, Thomas Spira, Jennifer Moore, John Sninsky, Gerald Schochetman, Alan R Lifson, Judith Galphin, Chin Yih Ou

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Abstract

The polymerase chain reaction (PCR) and reverse transcription were used to assess human immunodeficiency virus type 1 (HIV1) RNA expression in peripheral blood mononuclear cell samples from seropositive subjects. HIV RNA was detected from seropositive subjects who had no symptoms, lymphadenopathy syndrome, and acquired immunodeficiency syndrome. DNA PCR of the samples used for RNA extraction showed that seventeen of eighteen (94%) contained HIV proviral DNA. Eleven (65%) of the seventeen DNA-positive samples were also positive for HIV RNA, including samples from four patients undergoing antiviral drug treatment. Serum HIV antigen assays detected only six (32%) of the nineteen PCR-positive samples. Owing to the speed and high sensitivity of PCR for HIV detection, this technique will be suitable for monitoring antiviral therapy and the virus load of people with HIV infections.

Original language	English (US)
Pages (from-to)	596-599
Number of pages	4
Journal	The Lancet
Volume	332
Issue number	8611
DOIs	https://doi.org/10.1016/S0140-6736(88)90639-3
State	Published - Sep 10 1988
Externally published	Yes

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title = "DIRECT DETECTION OF HIV RNA EXPRESSION IN SEROPOSITIVE SUBJECTS",

abstract = "The polymerase chain reaction (PCR) and reverse transcription were used to assess human immunodeficiency virus type 1 (HIV1) RNA expression in peripheral blood mononuclear cell samples from seropositive subjects. HIV RNA was detected from seropositive subjects who had no symptoms, lymphadenopathy syndrome, and acquired immunodeficiency syndrome. DNA PCR of the samples used for RNA extraction showed that seventeen of eighteen (94%) contained HIV proviral DNA. Eleven (65%) of the seventeen DNA-positive samples were also positive for HIV RNA, including samples from four patients undergoing antiviral drug treatment. Serum HIV antigen assays detected only six (32%) of the nineteen PCR-positive samples. Owing to the speed and high sensitivity of PCR for HIV detection, this technique will be suitable for monitoring antiviral therapy and the virus load of people with HIV infections.",

author = "Clyde Hart and Thomas Spira and Jennifer Moore and John Sninsky and Gerald Schochetman and Lifson, {Alan R} and Judith Galphin and Ou, {Chin Yih}",

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T1 - DIRECT DETECTION OF HIV RNA EXPRESSION IN SEROPOSITIVE SUBJECTS

AU - Hart, Clyde

AU - Spira, Thomas

AU - Moore, Jennifer

AU - Sninsky, John

AU - Schochetman, Gerald

AU - Lifson, Alan R

AU - Galphin, Judith

AU - Ou, Chin Yih

PY - 1988/9/10

Y1 - 1988/9/10

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AB - The polymerase chain reaction (PCR) and reverse transcription were used to assess human immunodeficiency virus type 1 (HIV1) RNA expression in peripheral blood mononuclear cell samples from seropositive subjects. HIV RNA was detected from seropositive subjects who had no symptoms, lymphadenopathy syndrome, and acquired immunodeficiency syndrome. DNA PCR of the samples used for RNA extraction showed that seventeen of eighteen (94%) contained HIV proviral DNA. Eleven (65%) of the seventeen DNA-positive samples were also positive for HIV RNA, including samples from four patients undergoing antiviral drug treatment. Serum HIV antigen assays detected only six (32%) of the nineteen PCR-positive samples. Owing to the speed and high sensitivity of PCR for HIV detection, this technique will be suitable for monitoring antiviral therapy and the virus load of people with HIV infections.

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DIRECT DETECTION OF HIV RNA EXPRESSION IN SEROPOSITIVE SUBJECTS

Abstract

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