DIRECT DETECTION OF HIV RNA EXPRESSION IN SEROPOSITIVE SUBJECTS

Clyde Hart, Thomas Spira, Jennifer Moore, John Sninsky, Gerald Schochetman, Alan R Lifson, Judith Galphin, Chin Yih Ou

Research output: Contribution to journalArticlepeer-review

106 Scopus citations

Abstract

The polymerase chain reaction (PCR) and reverse transcription were used to assess human immunodeficiency virus type 1 (HIV1) RNA expression in peripheral blood mononuclear cell samples from seropositive subjects. HIV RNA was detected from seropositive subjects who had no symptoms, lymphadenopathy syndrome, and acquired immunodeficiency syndrome. DNA PCR of the samples used for RNA extraction showed that seventeen of eighteen (94%) contained HIV proviral DNA. Eleven (65%) of the seventeen DNA-positive samples were also positive for HIV RNA, including samples from four patients undergoing antiviral drug treatment. Serum HIV antigen assays detected only six (32%) of the nineteen PCR-positive samples. Owing to the speed and high sensitivity of PCR for HIV detection, this technique will be suitable for monitoring antiviral therapy and the virus load of people with HIV infections.

Original languageEnglish (US)
Pages (from-to)596-599
Number of pages4
JournalThe Lancet
Volume332
Issue number8611
DOIs
StatePublished - Sep 10 1988
Externally publishedYes

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