Direct cross-linking of 125I-labeled glucagon to its membrane receptor by UV irradiation

V. Iwanij, K. C. Hur

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29 Scopus citations

Abstract

125I-labeled glucagon was directly cross-linked to its receptor in isolated liver plasma membranes and on the surface of intact hepatocytes, by using a UV irradiation procedure. This investigation resulted in the identification of a glucagon-receptor complex of apparent M(r) 62,000. The specificity of labeling was shown by the interference of unlabeled hormone at physiological concentration with incorporation of radioactive glucagon into the 62,000 M(r) species. The receptor behaved as a typical integral membrane protein: it was not released by extraction with lithium diiodosalicylate or at basic pH but was solubilized by digitonin treatment. Reduction of the receptor polypeptide with dithiothreitol resulted in a decrease in its electrophoretic mobility, suggesting the presence of intramolecular disulfide bonds. Soluble glucagon-receptor complexes adsorbed to Con A-Sepharose and could be eluted with methyl α-D-mannoside, indicating that the receptor molecule is a glycoprotein. Treatment of glucagon-labeled liver plasma membrane with endoglycosidase F resulted in the appearance of four intermediate species, indicating that glucagon receptor contains at least four N-linked oligosaccharide chains.

Original languageEnglish (US)
Pages (from-to)325-329
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number2
DOIs
StatePublished - Jan 1 1985

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