Dioxin induces transforming growth factor-α in human keratinocytes

E. J. Choi, D. G. Toscano, J. A. Ryan, N. Riedel, W. A. Toscano

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2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a widespread environmental toxicant, is a tumor promoter that induces hyperplasia in epithelial cells. Exposure of cultured human keratinocytes to TCDD, resulted in a time-dependent dioxin-specific Ah receptor-mediated release of transforming growth factor-α (TGF-α) into the culture medium. Cultures exposed to TCDD showed a rate of TGF-α secretion into the medium of about 80 fmol/ml/day, as well as a 3- to 6-fold increase in TGF-α mRNA expression. Increased production of TGF-α in human keratinocytes exposed to TCDD demonstrates a modulation of autocrine regulation in those cells. These results suggest that induction of TGF-α could be an important part of the mechanism of dioxin-mediated toxicity and tumor promotion.

Original languageEnglish (US)
Pages (from-to)9591-9597
Number of pages7
JournalJournal of Biological Chemistry
Issue number15
StatePublished - 1991

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    Choi, E. J., Toscano, D. G., Ryan, J. A., Riedel, N., & Toscano, W. A. (1991). Dioxin induces transforming growth factor-α in human keratinocytes. Journal of Biological Chemistry, 266(15), 9591-9597.