Dilution technique for isolation of Haemophilus from swine lungs collected at slaughter

C. Pijoan, R. B. Morrison, H. D. Hilley

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A total of 307 lungs obtained from a slaughterhouse were cultured by a dilution technique for the isolation of Haemophilus spp. The technique consisted of performing serial (10-fold) dilutions of the tissue samples to a dilution of 10-5. Two selective media were used. L broth consisted of a basal brain heart infusion broth containing 5% horse serum, 5% yeast extract, and 100 μg of NAD and 0.5 μg of lincomycin per ml. L-B broth was identical to L broth, except 1.5 μg of bacitracin per ml was included. The broths were incubated overnight and then plated onto blood agar. A total of 83 (27%) isolates were obtained, and both media proved to be necessary, as a proportion of isolates grew in one of the media employed but not in the other. Of the isolates, 66.3% were urease positive and most of these (98%) were classified as 'minor group' strains. Urease-negative strains (27.7%) were classified as Haemophilus parasuis.

Original languageEnglish (US)
Pages (from-to)143-145
Number of pages3
JournalJournal of clinical microbiology
Volume18
Issue number1
StatePublished - Jan 1 1983

Fingerprint Dive into the research topics of 'Dilution technique for isolation of Haemophilus from swine lungs collected at slaughter'. Together they form a unique fingerprint.

Cite this