Differentiation of epidermal keratinocytes from human embryonic stem cells

Fahad K. Kidwai, Tong Cao, Kai Lu

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations


For many years, cell therapies have been hampered by limited availability and inter-batch variability of primary cells. Human embryonic stem cell (hESC) can give rise to specialized cells like keratinocytes and recently emerged as a virtually unlimited source of potential therapeutic cells. However, xenogeneic components in differentiation cocktails have been limiting the clinical potential of hESC-derived keratinocytes (hESCs-Kert). Here, we demonstrated efficient differentiation of H9 human embryonic stem cells (H9-hESCs) into keratinocytes (H9-KertACC) in an autogenic co-culture system. We used activin as the main factor to induce keratinocyte differentiation. H9-Kert ACC expressed keratinocyte markers at mRNA and protein levels. Establishment of such animal-free microenvironment for keratinocyte differentiation will accelerate potential clinical application of hESCs.

Original languageEnglish (US)
Title of host publicationEpidermal Cells
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages10
ISBN (Print)9781493912230
StatePublished - Jan 1 2014

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Activin
  • Co-culture
  • Differentiation
  • Epidermal
  • Human embryonic stem cell
  • Keratinocyte
  • Tissue engineering
  • Xeno-free


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