Differentiation of avian adenovirus type-II strains by restriction endonuclease fingerprinting.

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Abstract

Three serologically indistinguishable viruses from the avian adenovirus type-II splenomegaly virus of chickens, marble spleen disease virus of pheasants, and hemorrhagic enteritis virus of turkeys, were analyzed by restriction endonuclease fingerprinting. The DNA from these viruses were examined with 6 restriction endonucleases (Bgl II, EcoRI, HindIII, Hha I, Xho I, and BamHI). Markedly different DNA cleavage patterns were found in these virus isolates with all the 5 enzymes, except with BamHI, suggesting genetic differences between isolates of adenovirus type II. Restriction endonuclease analyses were found to provide a method for distinguishing genetically different, and yet serologically similar, strains of avian adenovirus type II.

Original languageEnglish (US)
Pages (from-to)1466-1470
Number of pages5
JournalAmerican journal of veterinary research
Volume50
Issue number9
StatePublished - Sep 1989

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